Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli

Yuzhen Zhou, Weidong Zhu, Padmanetra S. Bellur, Dustin Rewinkel, Donald F Becker

Research output: Contribution to journalReview article

28 Citations (Scopus)

Abstract

The control of gene expression by enzymes provides a direct pathway for cells to respond to fluctuations in metabolites and nutrients. One example is the proline utilization A (PutA) protein from Escherichia coli. PutA is a membrane-associated enzyme that catalyzes the oxidation of l-proline to glutamate using a flavin containing proline dehydrogenase domain and a NAD + dependent Δ1-pyrroline-5-carboxylate dehydrogenase domain. In some Gram-negative bacteria such as E. coli, PutA is also endowed with a ribbon-helix-helix DNA-binding domain and acts as a transcriptional repressor of the proline utilization genes. PutA switches between transcriptional repressor and enzymatic functions in response to proline availability. Molecular insights into the redox-based mechanism of PutA functional switching from recent studies are reviewed. In addition, new results from cell-based transcription assays are presented which correlate PutA membrane localization with put gene expression levels. General membrane localization of PutA, however, is not sufficient to activate the put genes.

Original languageEnglish (US)
Pages (from-to)711-718
Number of pages8
JournalAmino Acids
Volume35
Issue number4
DOIs
StatePublished - Nov 1 2008

Fingerprint

Escherichia coli Proteins
Proline
Metabolism
Gene expression
Gene Expression
Membranes
1-Pyrroline-5-Carboxylate Dehydrogenase
Proline Oxidase
Genes
Enzymes
Transcription
Metabolites
Gram-Negative Bacteria
NAD
Escherichia coli
Nutrients
Oxidation-Reduction
Glutamic Acid
Assays
Bacteria

Keywords

  • DNA-binding
  • Membrane-binding
  • Multifunctional enzyme
  • Proline utilization
  • PutA
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli. / Zhou, Yuzhen; Zhu, Weidong; Bellur, Padmanetra S.; Rewinkel, Dustin; Becker, Donald F.

In: Amino Acids, Vol. 35, No. 4, 01.11.2008, p. 711-718.

Research output: Contribution to journalReview article

Zhou, Yuzhen ; Zhu, Weidong ; Bellur, Padmanetra S. ; Rewinkel, Dustin ; Becker, Donald F. / Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli. In: Amino Acids. 2008 ; Vol. 35, No. 4. pp. 711-718.
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