Direct involvemnet of the isotype- specific C- terminus of β tubulin in ciliary beating

Julia Vent, Todd A Wyatt, David D. Smith, Asok Banerjee, Richard F. Ludueña, Joseph Harold Sisson, Richard Hallworth

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In previous studies in Drosophila, Nielsen et al. hypothesized that the β tubulin C-terminal axonemal motif 'EGEFXXX', where X is an acidic amino acid, is required for ciliary function and assembly (Nielsen et al., 2001, Curr. Biol. 11, 529-533). This motif is present in some but not all mammalian β tubulin isotypes. We therefore investigated whether this motif is important in ciliary function in mammals. In a preparation of isolated, ATP-reactivated bovine tracheal cilia, we found that monoclonal antibodies directed against the C-terminus of βI, βIV and βV tubulin blocked ciliary beating in a concentration dependent manner. Antibodies against other epitopes of β tubulin were ineffective, as were antibodies against α tubulin. Peptides consisting of the axonemal motif and motif-like sequences of these isotypes blocked ciliary beating. These results suggest that the axonemal motif sequences of βI, βIV and βV tubulin are essential for ciliary function. Peptides consisting of corresponding C-terminal sequences in α tubulin isotypes were also ineffective in blocking ciliary beating, which suggests that the C-terminus of α tubulin is not directly involved in cilia function in mammals.

Original languageEnglish (US)
Pages (from-to)4333-4341
Number of pages9
JournalJournal of cell science
Volume118
Issue number19
DOIs
StatePublished - Oct 1 2005

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Tubulin
Cilia
Mammals
Acidic Amino Acids
Peptides
Antibodies
Drosophila
Epitopes
Adenosine Triphosphate
Monoclonal Antibodies

Keywords

  • Axonemal function
  • Cilia
  • Ciliary beat frequency
  • β Tubulin isotype

ASJC Scopus subject areas

  • Cell Biology

Cite this

Direct involvemnet of the isotype- specific C- terminus of β tubulin in ciliary beating. / Vent, Julia; Wyatt, Todd A; Smith, David D.; Banerjee, Asok; Ludueña, Richard F.; Sisson, Joseph Harold; Hallworth, Richard.

In: Journal of cell science, Vol. 118, No. 19, 01.10.2005, p. 4333-4341.

Research output: Contribution to journalArticle

Vent, Julia ; Wyatt, Todd A ; Smith, David D. ; Banerjee, Asok ; Ludueña, Richard F. ; Sisson, Joseph Harold ; Hallworth, Richard. / Direct involvemnet of the isotype- specific C- terminus of β tubulin in ciliary beating. In: Journal of cell science. 2005 ; Vol. 118, No. 19. pp. 4333-4341.
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