Direct inhibition of murine B lymphocyte differentiation by 12-O-tetradecanoylphorbol-13-acetate

Loretta D. Schuman, Thomas M. Petro, C. Stuart Baxter

Research output: Contribution to journalArticle

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Abstract

The tumor-promoting agent, 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibits the humoral immune response of lymphocytes to antigen. To test the hypothesis that this inhibition is due to a direct effect upon B lymphocytes, splenic lymphocytes or murine B lymphocytes, enriched by 'panning' splenic lymphocytes onto anti-IgM-coated petri dishes, were immunized in vitro with the thymus/accessory cell-independent antigen trinitrophenyl lipopolysaccharide (TNP-LPS) with or without TPA. The number of anti-TNP antibody-forming cells present in both lymphocyte populations after 5 days was almost completely inhibited to the same degree by TPA. These data unambiguously show that TPA can directly inhibit the differentiation of B lymphocytes to antibody-forming cells.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalCancer Letters
Volume34
Issue number2
DOIs
StatePublished - Feb 1987

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Tetradecanoylphorbol Acetate
B-Lymphocytes
Lymphocytes
Antigens
Humoral Immunity
Thymus Gland
Anti-Idiotypic Antibodies
Antibodies
Population
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Direct inhibition of murine B lymphocyte differentiation by 12-O-tetradecanoylphorbol-13-acetate. / Schuman, Loretta D.; Petro, Thomas M.; Baxter, C. Stuart.

In: Cancer Letters, Vol. 34, No. 2, 02.1987, p. 157-163.

Research output: Contribution to journalArticle

Schuman, Loretta D. ; Petro, Thomas M. ; Baxter, C. Stuart. / Direct inhibition of murine B lymphocyte differentiation by 12-O-tetradecanoylphorbol-13-acetate. In: Cancer Letters. 1987 ; Vol. 34, No. 2. pp. 157-163.
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