Direct analysis of RNA transcripts in electroporated carrot protoplasts

Elizabeth E. Murray, Wallace G Buchholz, Benjamin Bowen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We describe a method for direct analysis of RNA transcribed from DNA introduced into carrot cells by electroporation. Octopine synthase RNA transcribed from the plasmid p35SOcs was detected in total and poly A+ RNA on Northern blots and in RNA protection assays. The highest level of octopine synthase transcript was detected at approximately 8 hrs post-electroporation, although RNA could still be detected after 48 hrs. This method allows detection of foreign gene expression in a plant system and bypasses the need for reporter genes.

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
JournalPlant Cell Reports
Volume9
Issue number3
DOIs
StatePublished - Jul 1 1990

Fingerprint

messenger RNA
carrots
protoplasts
RNA
octopine
electroporation
reporter genes
Northern blotting
plasmids
gene expression
DNA
assays
methodology
cells

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Direct analysis of RNA transcripts in electroporated carrot protoplasts. / Murray, Elizabeth E.; Buchholz, Wallace G; Bowen, Benjamin.

In: Plant Cell Reports, Vol. 9, No. 3, 01.07.1990, p. 129-132.

Research output: Contribution to journalArticle

Murray, Elizabeth E. ; Buchholz, Wallace G ; Bowen, Benjamin. / Direct analysis of RNA transcripts in electroporated carrot protoplasts. In: Plant Cell Reports. 1990 ; Vol. 9, No. 3. pp. 129-132.
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