Differentiation of two bovine lentiviruses by a monoclonal antibody on the basis of epitope specificity

L. Zheng, S. Zhang, C. Wood, S. Kapil, G. E. Wilcox, T. A. Loughin, H. C. Minocha

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bovine immunodeficiency virus (BIV) and Jembrana disease virus (JDV) are bovine lentiviruses that are closely related genetically. A recombinant fusion protein containing the capsid protein of BIV expressed in Escherichia coli was used to immunize mice and produce monoclonal antibodies. Six hybridomas specific for BIV capsid protein were identified, and one antibody, designated 10H1, was characterized further. Competitive binding assays were performed to analyze the topography of antigenic determinants by enzyme-linked immunosorbent assay and demonstrated the existence of at least three distinct antigenic determinants on capsid protein. The monoclonal antibody reacted specifically with both BIV capsid and the recombinant fusion protein in Western immunoblot analyses. However, it did not react with the recombinant capsid fusion protein of JDV, indicating that BIV contains at least one unique epitope in the capsid protein that is absent in JDV. Further mapping of the epitope by chemical cleavage analysis identified that the epitope is located at the 6.4-kDa N terminus of the 29-kDa capsid protein. This monoclonal antibody assay will be valuable for distinguishing the two closely related lentiviruses by Western blotting.

Original languageEnglish (US)
Pages (from-to)283-287
Number of pages5
JournalClinical and diagnostic laboratory immunology
Volume8
Issue number2
DOIs
StatePublished - Jan 1 2001

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Bovine Lentiviruses
Bovine Immunodeficiency Virus
Capsid Proteins
Viruses
Epitopes
Monoclonal Antibodies
Recombinant Fusion Proteins
Virus Diseases
Assays
Western Blotting
Epitope Mapping
Lentivirus
Competitive Binding
Capsid
Hybridomas
Immunosorbents
Enzyme-Linked Immunosorbent Assay
Topography
Escherichia coli
Fusion reactions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Differentiation of two bovine lentiviruses by a monoclonal antibody on the basis of epitope specificity. / Zheng, L.; Zhang, S.; Wood, C.; Kapil, S.; Wilcox, G. E.; Loughin, T. A.; Minocha, H. C.

In: Clinical and diagnostic laboratory immunology, Vol. 8, No. 2, 01.01.2001, p. 283-287.

Research output: Contribution to journalArticle

Zheng, L. ; Zhang, S. ; Wood, C. ; Kapil, S. ; Wilcox, G. E. ; Loughin, T. A. ; Minocha, H. C. / Differentiation of two bovine lentiviruses by a monoclonal antibody on the basis of epitope specificity. In: Clinical and diagnostic laboratory immunology. 2001 ; Vol. 8, No. 2. pp. 283-287.
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