Differentiation of islet cells in long-term culture

Bruno M. Schmied, Guozhen Liu, Hosei Matsuzaki, Alexis Ulrich, Sara Hernberg, Mary Pat Moyer, Lemont Weide, Leo Murphy, Surinder Kumar Batra, Parviz M. Pour

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Our previous studies in the hamster pancreatic cancer model have shown that exocrine pancreatic cancer arises from ductal/ductular cells, as well as from within the islets, most probably from islet precursor (stem) cells. To identify and characterize these cells, we established a long-term culture from isolated hamster islets and investigated their growth, differentiation, and expression of biomarkers. Islets maintained their original form and structure within the first 14 days in culture. However, beginning at day 7, ductular structures began to form within the islets. At day 21 in culture, acinar cells, intermediary cells, oncocytes, and cells comparable to pancreatic hepatocytes also appeared between ductular and endocrine cells. The number of duct-like cells gradually increased, whereas the number of hormone-producing cells decreased. After 35 days in culture, the exocrine cells disappeared, and undifferentiated cells formed a monolayer. These cells expressed cytokeratins, α1-antitrypsin, transforming growth factor-α, epidermal growth factor receptor, carbonic anhydrase II, vimentin, laminin, and showed binding to tomato lectin and Phaseolus vulgaris leukoagglutinin. They did not express the regulatory transcriptional factors; insulin- promoting factor 1, NKx6.1, Pax6, and NeuroD. The results thus indicate that islet cells have potential to form exocrine cells. At present, it is not clear whether these cells originate from preexisting stem cells or from transdifferentiated islet cells.

Original languageEnglish (US)
Pages (from-to)337-347
Number of pages11
JournalPancreas
Volume20
Issue number4
DOIs
StatePublished - May 1 2000

Fingerprint

Islets of Langerhans
Pancreatic Neoplasms
Cricetinae
Stem Cells
Oxyphil Cells
Carbonic Anhydrase II
Endocrine Cells
Acinar Cells
Transforming Growth Factors
Laminin
Vimentin
Keratins
Epidermal Growth Factor Receptor
Hepatocytes
Cell Culture Techniques
Biomarkers
Hormones
Insulin
Growth

Keywords

  • Acinar
  • Differentiation
  • Ductular cells
  • Hamster
  • Intermediary
  • Islets

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Schmied, B. M., Liu, G., Matsuzaki, H., Ulrich, A., Hernberg, S., Moyer, M. P., ... Pour, P. M. (2000). Differentiation of islet cells in long-term culture. Pancreas, 20(4), 337-347. https://doi.org/10.1097/00006676-200005000-00002

Differentiation of islet cells in long-term culture. / Schmied, Bruno M.; Liu, Guozhen; Matsuzaki, Hosei; Ulrich, Alexis; Hernberg, Sara; Moyer, Mary Pat; Weide, Lemont; Murphy, Leo; Batra, Surinder Kumar; Pour, Parviz M.

In: Pancreas, Vol. 20, No. 4, 01.05.2000, p. 337-347.

Research output: Contribution to journalArticle

Schmied, BM, Liu, G, Matsuzaki, H, Ulrich, A, Hernberg, S, Moyer, MP, Weide, L, Murphy, L, Batra, SK & Pour, PM 2000, 'Differentiation of islet cells in long-term culture', Pancreas, vol. 20, no. 4, pp. 337-347. https://doi.org/10.1097/00006676-200005000-00002
Schmied BM, Liu G, Matsuzaki H, Ulrich A, Hernberg S, Moyer MP et al. Differentiation of islet cells in long-term culture. Pancreas. 2000 May 1;20(4):337-347. https://doi.org/10.1097/00006676-200005000-00002
Schmied, Bruno M. ; Liu, Guozhen ; Matsuzaki, Hosei ; Ulrich, Alexis ; Hernberg, Sara ; Moyer, Mary Pat ; Weide, Lemont ; Murphy, Leo ; Batra, Surinder Kumar ; Pour, Parviz M. / Differentiation of islet cells in long-term culture. In: Pancreas. 2000 ; Vol. 20, No. 4. pp. 337-347.
@article{362c5a68765341fd88f8d7d5306f6f66,
title = "Differentiation of islet cells in long-term culture",
abstract = "Our previous studies in the hamster pancreatic cancer model have shown that exocrine pancreatic cancer arises from ductal/ductular cells, as well as from within the islets, most probably from islet precursor (stem) cells. To identify and characterize these cells, we established a long-term culture from isolated hamster islets and investigated their growth, differentiation, and expression of biomarkers. Islets maintained their original form and structure within the first 14 days in culture. However, beginning at day 7, ductular structures began to form within the islets. At day 21 in culture, acinar cells, intermediary cells, oncocytes, and cells comparable to pancreatic hepatocytes also appeared between ductular and endocrine cells. The number of duct-like cells gradually increased, whereas the number of hormone-producing cells decreased. After 35 days in culture, the exocrine cells disappeared, and undifferentiated cells formed a monolayer. These cells expressed cytokeratins, α1-antitrypsin, transforming growth factor-α, epidermal growth factor receptor, carbonic anhydrase II, vimentin, laminin, and showed binding to tomato lectin and Phaseolus vulgaris leukoagglutinin. They did not express the regulatory transcriptional factors; insulin- promoting factor 1, NKx6.1, Pax6, and NeuroD. The results thus indicate that islet cells have potential to form exocrine cells. At present, it is not clear whether these cells originate from preexisting stem cells or from transdifferentiated islet cells.",
keywords = "Acinar, Differentiation, Ductular cells, Hamster, Intermediary, Islets",
author = "Schmied, {Bruno M.} and Guozhen Liu and Hosei Matsuzaki and Alexis Ulrich and Sara Hernberg and Moyer, {Mary Pat} and Lemont Weide and Leo Murphy and Batra, {Surinder Kumar} and Pour, {Parviz M.}",
year = "2000",
month = "5",
day = "1",
doi = "10.1097/00006676-200005000-00002",
language = "English (US)",
volume = "20",
pages = "337--347",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Differentiation of islet cells in long-term culture

AU - Schmied, Bruno M.

AU - Liu, Guozhen

AU - Matsuzaki, Hosei

AU - Ulrich, Alexis

AU - Hernberg, Sara

AU - Moyer, Mary Pat

AU - Weide, Lemont

AU - Murphy, Leo

AU - Batra, Surinder Kumar

AU - Pour, Parviz M.

PY - 2000/5/1

Y1 - 2000/5/1

N2 - Our previous studies in the hamster pancreatic cancer model have shown that exocrine pancreatic cancer arises from ductal/ductular cells, as well as from within the islets, most probably from islet precursor (stem) cells. To identify and characterize these cells, we established a long-term culture from isolated hamster islets and investigated their growth, differentiation, and expression of biomarkers. Islets maintained their original form and structure within the first 14 days in culture. However, beginning at day 7, ductular structures began to form within the islets. At day 21 in culture, acinar cells, intermediary cells, oncocytes, and cells comparable to pancreatic hepatocytes also appeared between ductular and endocrine cells. The number of duct-like cells gradually increased, whereas the number of hormone-producing cells decreased. After 35 days in culture, the exocrine cells disappeared, and undifferentiated cells formed a monolayer. These cells expressed cytokeratins, α1-antitrypsin, transforming growth factor-α, epidermal growth factor receptor, carbonic anhydrase II, vimentin, laminin, and showed binding to tomato lectin and Phaseolus vulgaris leukoagglutinin. They did not express the regulatory transcriptional factors; insulin- promoting factor 1, NKx6.1, Pax6, and NeuroD. The results thus indicate that islet cells have potential to form exocrine cells. At present, it is not clear whether these cells originate from preexisting stem cells or from transdifferentiated islet cells.

AB - Our previous studies in the hamster pancreatic cancer model have shown that exocrine pancreatic cancer arises from ductal/ductular cells, as well as from within the islets, most probably from islet precursor (stem) cells. To identify and characterize these cells, we established a long-term culture from isolated hamster islets and investigated their growth, differentiation, and expression of biomarkers. Islets maintained their original form and structure within the first 14 days in culture. However, beginning at day 7, ductular structures began to form within the islets. At day 21 in culture, acinar cells, intermediary cells, oncocytes, and cells comparable to pancreatic hepatocytes also appeared between ductular and endocrine cells. The number of duct-like cells gradually increased, whereas the number of hormone-producing cells decreased. After 35 days in culture, the exocrine cells disappeared, and undifferentiated cells formed a monolayer. These cells expressed cytokeratins, α1-antitrypsin, transforming growth factor-α, epidermal growth factor receptor, carbonic anhydrase II, vimentin, laminin, and showed binding to tomato lectin and Phaseolus vulgaris leukoagglutinin. They did not express the regulatory transcriptional factors; insulin- promoting factor 1, NKx6.1, Pax6, and NeuroD. The results thus indicate that islet cells have potential to form exocrine cells. At present, it is not clear whether these cells originate from preexisting stem cells or from transdifferentiated islet cells.

KW - Acinar

KW - Differentiation

KW - Ductular cells

KW - Hamster

KW - Intermediary

KW - Islets

UR - http://www.scopus.com/inward/record.url?scp=17644440519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17644440519&partnerID=8YFLogxK

U2 - 10.1097/00006676-200005000-00002

DO - 10.1097/00006676-200005000-00002

M3 - Article

VL - 20

SP - 337

EP - 347

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 4

ER -