Differentiation of embryonic stem cells to retinal cells in vitro.

Xing Zhao, Jianuo Liu, Iqbal Ahmad

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Currently, there is no effective treatment for photoreceptor degeneration, the most common cause of blindness caused by diseases like retinitis pigmentosa, age-related macular degeneration, and diabetic retinopathy. Two promising approaches include cell therapy to replace degenerating cells and neuroprotection to rescue affected cells from premature death. Determination of the potential of embryonic stem (ES) cells to differentiate into photoreceptors will provide reagents for both approaches. First, neural progenitors with retinal potential will be available in unlimited supply to test the efficacy of cell therapy; second, the controlled differentiation of ES cells into photoreceptors, in addition to providing cells to replace degenerating photoreceptors, will offer a robust in vitro model of photoreceptor differentiation for better understanding of degenerative processes and screening of neuroprotective drugs/reagents. In addition, it will allow the identification of genes (gene discovery) that play critical roles in photoreceptor differentiation and degeneration. Here, we describe the protocol to promote differentiation of the mouse ES cell-derived neural progenitors into retinal cells, specifically the rod photoreceptors.

Original languageEnglish (US)
Pages (from-to)401-416
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume330
StatePublished - 2006

Fingerprint

Embryonic Stem Cells
Cell- and Tissue-Based Therapy
Retinal Rod Photoreceptor Cells
Premature Mortality
Retinitis Pigmentosa
Macular Degeneration
Genetic Association Studies
Neuroprotective Agents
Diabetic Retinopathy
Blindness
Cell Death
Genes
In Vitro Techniques
Therapeutics
Neuroprotection
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Differentiation of embryonic stem cells to retinal cells in vitro. / Zhao, Xing; Liu, Jianuo; Ahmad, Iqbal.

In: Methods in molecular biology (Clifton, N.J.), Vol. 330, 2006, p. 401-416.

Research output: Contribution to journalArticle

@article{93a9abfa16884911a20d4a1777a093a6,
title = "Differentiation of embryonic stem cells to retinal cells in vitro.",
abstract = "Currently, there is no effective treatment for photoreceptor degeneration, the most common cause of blindness caused by diseases like retinitis pigmentosa, age-related macular degeneration, and diabetic retinopathy. Two promising approaches include cell therapy to replace degenerating cells and neuroprotection to rescue affected cells from premature death. Determination of the potential of embryonic stem (ES) cells to differentiate into photoreceptors will provide reagents for both approaches. First, neural progenitors with retinal potential will be available in unlimited supply to test the efficacy of cell therapy; second, the controlled differentiation of ES cells into photoreceptors, in addition to providing cells to replace degenerating photoreceptors, will offer a robust in vitro model of photoreceptor differentiation for better understanding of degenerative processes and screening of neuroprotective drugs/reagents. In addition, it will allow the identification of genes (gene discovery) that play critical roles in photoreceptor differentiation and degeneration. Here, we describe the protocol to promote differentiation of the mouse ES cell-derived neural progenitors into retinal cells, specifically the rod photoreceptors.",
author = "Xing Zhao and Jianuo Liu and Iqbal Ahmad",
year = "2006",
language = "English (US)",
volume = "330",
pages = "401--416",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Differentiation of embryonic stem cells to retinal cells in vitro.

AU - Zhao, Xing

AU - Liu, Jianuo

AU - Ahmad, Iqbal

PY - 2006

Y1 - 2006

N2 - Currently, there is no effective treatment for photoreceptor degeneration, the most common cause of blindness caused by diseases like retinitis pigmentosa, age-related macular degeneration, and diabetic retinopathy. Two promising approaches include cell therapy to replace degenerating cells and neuroprotection to rescue affected cells from premature death. Determination of the potential of embryonic stem (ES) cells to differentiate into photoreceptors will provide reagents for both approaches. First, neural progenitors with retinal potential will be available in unlimited supply to test the efficacy of cell therapy; second, the controlled differentiation of ES cells into photoreceptors, in addition to providing cells to replace degenerating photoreceptors, will offer a robust in vitro model of photoreceptor differentiation for better understanding of degenerative processes and screening of neuroprotective drugs/reagents. In addition, it will allow the identification of genes (gene discovery) that play critical roles in photoreceptor differentiation and degeneration. Here, we describe the protocol to promote differentiation of the mouse ES cell-derived neural progenitors into retinal cells, specifically the rod photoreceptors.

AB - Currently, there is no effective treatment for photoreceptor degeneration, the most common cause of blindness caused by diseases like retinitis pigmentosa, age-related macular degeneration, and diabetic retinopathy. Two promising approaches include cell therapy to replace degenerating cells and neuroprotection to rescue affected cells from premature death. Determination of the potential of embryonic stem (ES) cells to differentiate into photoreceptors will provide reagents for both approaches. First, neural progenitors with retinal potential will be available in unlimited supply to test the efficacy of cell therapy; second, the controlled differentiation of ES cells into photoreceptors, in addition to providing cells to replace degenerating photoreceptors, will offer a robust in vitro model of photoreceptor differentiation for better understanding of degenerative processes and screening of neuroprotective drugs/reagents. In addition, it will allow the identification of genes (gene discovery) that play critical roles in photoreceptor differentiation and degeneration. Here, we describe the protocol to promote differentiation of the mouse ES cell-derived neural progenitors into retinal cells, specifically the rod photoreceptors.

UR - http://www.scopus.com/inward/record.url?scp=33747396888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747396888&partnerID=8YFLogxK

M3 - Article

C2 - 16846039

AN - SCOPUS:33747396888

VL - 330

SP - 401

EP - 416

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -