Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-λ1) in response to influenza a infection

Jieru Wang, Rebecca Oberley-Deegan, Shuanglin Wang, Mrinalini Nikrad, C. Joel Funk, Kevan L. Hartshorn, Robert J. Mason

Research output: Contribution to journalArticle

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Abstract

Alveolar type II epithelial cells (ATIIs) are one of the primary targets for influenza A pneumonia. The lack of a culture system for maintaining differentiated ATIIs hinders our understanding of pulmonary innate immunity during viral infection. We studied influenza A virus (IAV)-induced innate immune responses in differentiated primary human ATIIs and alveolar macrophages (AMs). Our results indicate that ATIIs, but not AMs, support productive IAV infection. Viral infection elicited strong inflammatory chemokine and cytokine responses in ATIIs, including secretion of IL-8, IL-6, MCP-1, RANTES, and MIP-1β, but not TNF-α, whereas AMs secreted TNF-α as well as other cytokines in response to infection. Wild-type virus A/PR/8/34 induced a greater cytokine response than reassortant PR/8 virus, A/Phil/82, despite similar levels of replication. IAV infection increased mRNA expression of IFN genes IFN-β, IL-29 (IFN-λ1), and IL-28A (IFN-λ2). The major IFN protein secreted by type II cells was IL-29 and ATIIs appear to be a major resource for production of IL-29. Administration of IL-29 and IFN-β before infection significantly reduced the release of infectious viral particles and CXC and CC chemokines. IL-29 treatment of type II cells induced mRNA expression of antiviral genes MX1, OAS, and ISG56 but not IFN-β. IL-29 induced a dose-dependent decrease of viral nucleoprotein and an increase of antiviral genes but not IFN-β. These results suggest that IL-29 exerts IFN-β-independent protection in type II cells through direct activation of antiviral genes during IAV infection.

Original languageEnglish (US)
Pages (from-to)1296-1304
Number of pages9
JournalJournal of Immunology
Volume182
Issue number3
StatePublished - Feb 1 2009

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Alveolar Epithelial Cells
Virus Diseases
Human Influenza
Antiviral Agents
Influenza A virus
Alveolar Macrophages
Infection
Cytokines
Innate Immunity
Viruses
Gene Expression
CXC Chemokines
Chemokine CCL5
CC Chemokines
Messenger RNA
Nucleoproteins
Interleukin-8
Interleukin-1
Chemokines
Virion

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Wang, J., Oberley-Deegan, R., Wang, S., Nikrad, M., Funk, C. J., Hartshorn, K. L., & Mason, R. J. (2009). Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-λ1) in response to influenza a infection. Journal of Immunology, 182(3), 1296-1304.

Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-λ1) in response to influenza a infection. / Wang, Jieru; Oberley-Deegan, Rebecca; Wang, Shuanglin; Nikrad, Mrinalini; Funk, C. Joel; Hartshorn, Kevan L.; Mason, Robert J.

In: Journal of Immunology, Vol. 182, No. 3, 01.02.2009, p. 1296-1304.

Research output: Contribution to journalArticle

Wang, J, Oberley-Deegan, R, Wang, S, Nikrad, M, Funk, CJ, Hartshorn, KL & Mason, RJ 2009, 'Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-λ1) in response to influenza a infection', Journal of Immunology, vol. 182, no. 3, pp. 1296-1304.
Wang, Jieru ; Oberley-Deegan, Rebecca ; Wang, Shuanglin ; Nikrad, Mrinalini ; Funk, C. Joel ; Hartshorn, Kevan L. ; Mason, Robert J. / Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-λ1) in response to influenza a infection. In: Journal of Immunology. 2009 ; Vol. 182, No. 3. pp. 1296-1304.
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