Differential role of psoriasin (S100A7) in estrogen receptor α positive and negative breast cancer cells occur through actin remodeling

Amita Sneh, Yadwinder S. Deol, Akaansha Ganju, Konstantin Shilo, Thomas J. Rosol, Mohd W Nasser, Ramesh K. Ganju

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Psoriasin (S100A7) is a calcium-binding protein that has shown to be highly expressed in high-grade ductal carcinoma in situ (DCIS) and a subset of invasive breast cancers. However, its role in invasion and metastasis is not very well known. In this study, we have shown that S100A7 differentially regulates epidermal growth factor (EGF)-induced cell migration and invasion in ERα- MDA-MB-231 cells and ERα+ MCF-7 and T47D breast cancer cells. Further signaling studies revealed that S100A7 enhances EGF-induced EGFR phosphorylation and actin remodeling that seems to favor lamellipodia formation in ERα- cells. In addition, S100A7 overexpression enhanced NF-κB-mediated matrix metalloproteinase-9 (MMP-9) secretion in MDA-MB-231 cells indicating its role in enhanced invasiveness. However, S100A7 overexpression inhibited migration and invasion of MCF-7 cells by inactivating Rac-1 pathway and MMP-9 secretion. Moreover, S100A7 overexpressing MDA-MB-231 cells showed enhanced metastasis compared to vector control in in vivo nude mice as detected by bioluminescence imaging. Our tissue microarray data also revealed predominant expression of S100A7 in ERα- metastatic carcinoma, especially in lymph node regions. Overall these studies suggest that S100A7 may enhance metastasis in ERα- breast cancer cells by a novel mechanism through regulation of actin cytoskeleton and MMP-9 secretion.

Original languageEnglish (US)
Pages (from-to)727-739
Number of pages13
JournalBreast Cancer Research and Treatment
Volume138
Issue number3
DOIs
StatePublished - Apr 1 2013

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Estrogen Receptors
Actins
Breast Neoplasms
Matrix Metalloproteinase 9
MCF-7 Cells
Neoplasm Metastasis
Epidermal Growth Factor
Pseudopodia
Calcium-Binding Proteins
Carcinoma, Intraductal, Noninfiltrating
Actin Cytoskeleton
Nude Mice
Cell Movement
Lymph Nodes
Phosphorylation
Carcinoma

Keywords

  • Actin remodeling
  • Breast cancer
  • Estrogen receptor-α
  • MMP-9
  • S100A7

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Differential role of psoriasin (S100A7) in estrogen receptor α positive and negative breast cancer cells occur through actin remodeling. / Sneh, Amita; Deol, Yadwinder S.; Ganju, Akaansha; Shilo, Konstantin; Rosol, Thomas J.; Nasser, Mohd W; Ganju, Ramesh K.

In: Breast Cancer Research and Treatment, Vol. 138, No. 3, 01.04.2013, p. 727-739.

Research output: Contribution to journalArticle

Sneh, Amita ; Deol, Yadwinder S. ; Ganju, Akaansha ; Shilo, Konstantin ; Rosol, Thomas J. ; Nasser, Mohd W ; Ganju, Ramesh K. / Differential role of psoriasin (S100A7) in estrogen receptor α positive and negative breast cancer cells occur through actin remodeling. In: Breast Cancer Research and Treatment. 2013 ; Vol. 138, No. 3. pp. 727-739.
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