Differential regulation of the transforming growth factor type-β2 gene promoter in embryonal carcinoma cells and their differentiated cells

David Lee Kelly, Michael A. O'Reilly, A Angie Rizzino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Previous studies have shown that EC cells do not express detectable levels of TGF-β2 or its mRNA until they differentiate. This suggested that differentiation influences the transcription of the TGF-β2 gene in this model system. To address this possibility, we have examined the activity of the TGF-β2 promoter in EC cells and their differentiated cells using gene constructs containing various portions of the TGF-β2 promoter inserted upstream of the reporter gene, chloramphenicol acetyltransferase (CAT). We determined that the level of CAT increases approximately ninefold when EC cells were induced to differentiate. Our studies also indicate that the TGF-β2 promoter contains at least two positive regulatory elements that are separated by a negative regulatory element. Finally, we have identified a CRE ATF-like site that appears to be responsible for a positive regulatory element located between -77 and -40.

Original languageEnglish (US)
Pages (from-to)172-175
Number of pages4
JournalDevelopmental Biology
Volume153
Issue number1
DOIs
StatePublished - Sep 1992

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Embryonal Carcinoma Stem Cells
Transforming Growth Factors
Chloramphenicol O-Acetyltransferase
Genes
Reporter Genes
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

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abstract = "Previous studies have shown that EC cells do not express detectable levels of TGF-β2 or its mRNA until they differentiate. This suggested that differentiation influences the transcription of the TGF-β2 gene in this model system. To address this possibility, we have examined the activity of the TGF-β2 promoter in EC cells and their differentiated cells using gene constructs containing various portions of the TGF-β2 promoter inserted upstream of the reporter gene, chloramphenicol acetyltransferase (CAT). We determined that the level of CAT increases approximately ninefold when EC cells were induced to differentiate. Our studies also indicate that the TGF-β2 promoter contains at least two positive regulatory elements that are separated by a negative regulatory element. Finally, we have identified a CRE ATF-like site that appears to be responsible for a positive regulatory element located between -77 and -40.",
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