Differential modulation of rod and cone calcium currents in tiger salamander retina by D2 dopamine receptors and cAMP

Jr Stella S.L., Wallace B Thoreson

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Synaptic transmission from vertebrate photoreceptors involves activation of L-type calcium currents (I(Ca)). Dopamine is an important circadian neuromodulator in the retina and photoreceptors possess D2 dopamine receptors. We examined modulation of I(Ca) by dopamine and cAMP in retinal slices and isolated cells of larval tiger salamander. Results show that dopamine and a D2 agonist, quinpirole, enhanced I(Ca) in rods and red-, blue- and UV-sensitive small single cones but inhibited I(Ca) in red-sensitive large single cones. A D1 agonist, SKF-38393, was without effect. Quinpirole effects were blocked by pertussis toxin (PTx) pretreatment indicating involvement of PTx-sensitive G-proteins. Like dopamine, inhibition of cAMP-dependent protein kinase (PKA) by Rp-cAMPS enhanced I(Ca) in rods and small single cones, but inhibited I(Ca) in large single cones. In contrast, forskolin and Sp-cAMPS, which stimulate PKA, inhibited I(Ca) in rods and small single cones but enhanced I(Ca) in large single cones. Sp-cAMPS also occluded effects of quinpirole. These results suggest that D2 receptors modulate I(Ca) via inhibition of cAMP. Differences among the responses of photoreceptors to cAMP are consistent with the possibility that small single cones and rods may possess different Ca2+ channel subtypes than large single cones. The results with dopamine and quinpirole showing inhibition of I(Ca) in large single cones and enhancement of rod I(Ca) were unexpected because previous studies have shown that dopamine suppresses rod inputs and enhances cone inputs into second-order neurons. The present results therefore indicate that the dopaminergic enhancement of cone inputs does not arise from modulation of photoreceptor I(Ca).

Original languageEnglish (US)
Pages (from-to)3537-3548
Number of pages12
JournalEuropean Journal of Neuroscience
Volume12
Issue number10
DOIs
StatePublished - Jan 1 2000

Fingerprint

Ambystoma
Vertebrate Photoreceptor Cells
Dopamine D2 Receptors
Quinpirole
Retina
Dopamine
Calcium
Pertussis Toxin
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Colforsin
Cyclic AMP-Dependent Protein Kinases
GTP-Binding Proteins
Synaptic Transmission
Protein Kinases
Neurotransmitter Agents
Neurons

Keywords

  • Calcium channel
  • Forskolin
  • Photoreceptors
  • Rp-cAMPS
  • Sp-cAMPS

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential modulation of rod and cone calcium currents in tiger salamander retina by D2 dopamine receptors and cAMP. / Stella S.L., Jr; Thoreson, Wallace B.

In: European Journal of Neuroscience, Vol. 12, No. 10, 01.01.2000, p. 3537-3548.

Research output: Contribution to journalArticle

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