Differential expression of linker histone variants in Euplotes crassus

S. Ray, C. Jahn, C. M. Tebeau, M. N. Larson, C. M. Price

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Two genes have been cloned from the ciliate Euplotes crassus that encode proteins with sequence similarity to the linker histones from a variety of organisms. One gene, H1-1, is present on a 1.3-kb macronuclear DNA molecule and encodes a 16.2-kDa protein. The second gene; H1-2, is present on a 0.7- kb DNA molecule and encodes an 18.8-kDa protein. Both H1-1 and H1-2 are expressed in vegetative cells, but the two genes exhibit very different patterns of expression during macronuclear development. H1-1 transcripts accumulate during conjugation and during the final rounds of DNA amplification. H1-2 transcripts accumulate after the onset of polytene chromosome formation and remain high throughout the remainder of macronuclear development. H1-1 is the major perchloric-acid-soluble protein from macronuclei. The pattern of gene expression and the macronuclear location of the H1-1 protein indicate that H1-1 is the predominant linker histone in vegetative macronuclei.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalGene
Volume231
Issue number1-2
DOIs
Publication statusPublished - Apr 29 1999

    Fingerprint

Keywords

  • Chromatin
  • Ciliate
  • Histone H1

ASJC Scopus subject areas

  • Genetics

Cite this