Differential expression of linker histone variants in Euplotes crassus

S. Ray, C. Jahn, C. M. Tebeau, Marilynn A Larson, C. M. Price

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two genes have been cloned from the ciliate Euplotes crassus that encode proteins with sequence similarity to the linker histones from a variety of organisms. One gene, H1-1, is present on a 1.3-kb macronuclear DNA molecule and encodes a 16.2-kDa protein. The second gene; H1-2, is present on a 0.7- kb DNA molecule and encodes an 18.8-kDa protein. Both H1-1 and H1-2 are expressed in vegetative cells, but the two genes exhibit very different patterns of expression during macronuclear development. H1-1 transcripts accumulate during conjugation and during the final rounds of DNA amplification. H1-2 transcripts accumulate after the onset of polytene chromosome formation and remain high throughout the remainder of macronuclear development. H1-1 is the major perchloric-acid-soluble protein from macronuclei. The pattern of gene expression and the macronuclear location of the H1-1 protein indicate that H1-1 is the predominant linker histone in vegetative macronuclei.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalGene
Volume231
Issue number1-2
DOIs
StatePublished - Apr 29 1999

Fingerprint

Euplotes
Histones
Macronucleus
Proteins
Genes
DNA
Polytene Chromosomes
Gene Expression

Keywords

  • Chromatin
  • Ciliate
  • Histone H1

ASJC Scopus subject areas

  • Genetics

Cite this

Differential expression of linker histone variants in Euplotes crassus. / Ray, S.; Jahn, C.; Tebeau, C. M.; Larson, Marilynn A; Price, C. M.

In: Gene, Vol. 231, No. 1-2, 29.04.1999, p. 15-20.

Research output: Contribution to journalArticle

Ray, S. ; Jahn, C. ; Tebeau, C. M. ; Larson, Marilynn A ; Price, C. M. / Differential expression of linker histone variants in Euplotes crassus. In: Gene. 1999 ; Vol. 231, No. 1-2. pp. 15-20.
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