Differential expression of CXCL12 and CXCR4 during human fetal neural progenitor cell differentiation

Hui Peng, Ryan Kolb, J. E. Kennedy, Jialin C Zheng

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Stromal cell-derived factor 1 alpha (SDF-1α, CXCL12) and its receptor CXCR4 play an important role in the central nervous system (CNS) development and adulthood by mediating cell migration, enhancing precursor cell proliferation, assisting in neuronal circuit formation, and possibly regulating migration during repair. The expression pattern of CXCR4 and CXCL12 during neurogenesis has not been thoroughly elucidated. In this study, we investigated the expression of CXCL12 and CXCR4 during neural progenitor cells (NPC) differentiation by microarray analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) using human fetal NPC as a model system. The production of CXCL12 was measured by enzyme-linked immunosorbent assay (ELISA). CXCR4 expression was determined by florescence-activated cell sorting (FACS) analysis, immunocytochemical staining, and CXCR4-mediated inhibition of cyclic AMP (cAMP) accumulation. Our data demonstrated that CXCR4 expression is significantly upregulated when NPC are differentiated into neuronal precursors, whereas CXCL12 is upregulated when differentiated into astrocytes. We also provide evidence that CXCR4 localization changes as neurons mature. In neuronal precursors, CXCR4 is localized in both neuronal processes and the cell body, whereas in mature neurons, it is primarily expressed on axons and dendrites. This differential expression of CXCR4 and CXCL12 may be important for the temporal regulation of neuronal migration and circuit formation during development and possibly in adult neurogenesis and repair.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalJournal of Neuroimmune Pharmacology
Volume2
Issue number3
DOIs
StatePublished - Sep 1 2007

Fingerprint

Cell Differentiation
Stem Cells
Neurogenesis
Tissue Array Analysis
CXCR4 Receptors
Neurons
Chemokine CXCL12
Dendrites
Reverse Transcriptase Polymerase Chain Reaction
Astrocytes
Cyclic AMP
Cell Movement
Axons
Central Nervous System
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Staining and Labeling
Cell Body

Keywords

  • Astrocytes
  • CXCL12
  • CXCR4
  • Neural precursor
  • Neural progenitor cells
  • Neurogenesis

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Differential expression of CXCL12 and CXCR4 during human fetal neural progenitor cell differentiation. / Peng, Hui; Kolb, Ryan; Kennedy, J. E.; Zheng, Jialin C.

In: Journal of Neuroimmune Pharmacology, Vol. 2, No. 3, 01.09.2007, p. 251-258.

Research output: Contribution to journalArticle

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