Differential expression and secretion of bovine serum amyloid A3 (SAA3) by mammary epithelial cells stimulated with prolactin or lipopolysaccharide

Marilynn A. Larson, Annika Weber, Allen T. Weber, Thomas L. McDonald

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Serum amyloid A (SAA) proteins were originally identified as prominent acute phase serum reactants synthesized predominately by hepatocytes in response to infection, inflammation and trauma. In this study, we report the differential expression and secretion of serum amyloid A3 (SAA3) by bovine mammary epithelial cells following stimulation with either prolactin (PRL) or lipopolysaccharide (LPS). Reverse transcription-polymerase chain reaction analysis of PRL or LPS induced bovine mammary epithelial cells resulted in the detection of only the mammary-derived Saa3 (M-Saa3) transcript. Two-dimensional immunoblot analyses of colostrum and milk from healthy cows, as well as conditioned medium from PRL or LPS stimulated bovine mammary epithelial cells confirmed the differential production and secretion of M-SAA3 while other SAA isoforms were not detected. These data indicate that the bovine Saa3 gene is regulated differently from the other Saa genes with regard to the site of and stimulus for expression, suggesting an important tissue-specific function for bovine M-SAA3 during lactation and mammary infection.

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume107
Issue number3-4
DOIs
StatePublished - Sep 15 2005

Fingerprint

amyloid
prolactin
Amyloid
Prolactin
lipopolysaccharides
breasts
Lipopolysaccharides
Breast
epithelial cells
Epithelial Cells
secretion
cattle
Serum Amyloid A Protein
Serum
Colostrum
Acute-Phase Proteins
Conditioned Culture Medium
Infection
colostrum
Lactation

Keywords

  • Bovine serum amyloid A3
  • Colostrum
  • Lipopolysaccharide
  • Mammary gland
  • Mastitis
  • Prolactin

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

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title = "Differential expression and secretion of bovine serum amyloid A3 (SAA3) by mammary epithelial cells stimulated with prolactin or lipopolysaccharide",
abstract = "Serum amyloid A (SAA) proteins were originally identified as prominent acute phase serum reactants synthesized predominately by hepatocytes in response to infection, inflammation and trauma. In this study, we report the differential expression and secretion of serum amyloid A3 (SAA3) by bovine mammary epithelial cells following stimulation with either prolactin (PRL) or lipopolysaccharide (LPS). Reverse transcription-polymerase chain reaction analysis of PRL or LPS induced bovine mammary epithelial cells resulted in the detection of only the mammary-derived Saa3 (M-Saa3) transcript. Two-dimensional immunoblot analyses of colostrum and milk from healthy cows, as well as conditioned medium from PRL or LPS stimulated bovine mammary epithelial cells confirmed the differential production and secretion of M-SAA3 while other SAA isoforms were not detected. These data indicate that the bovine Saa3 gene is regulated differently from the other Saa genes with regard to the site of and stimulus for expression, suggesting an important tissue-specific function for bovine M-SAA3 during lactation and mammary infection.",
keywords = "Bovine serum amyloid A3, Colostrum, Lipopolysaccharide, Mammary gland, Mastitis, Prolactin",
author = "Larson, {Marilynn A.} and Annika Weber and Weber, {Allen T.} and McDonald, {Thomas L.}",
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AU - Larson, Marilynn A.

AU - Weber, Annika

AU - Weber, Allen T.

AU - McDonald, Thomas L.

PY - 2005/9/15

Y1 - 2005/9/15

N2 - Serum amyloid A (SAA) proteins were originally identified as prominent acute phase serum reactants synthesized predominately by hepatocytes in response to infection, inflammation and trauma. In this study, we report the differential expression and secretion of serum amyloid A3 (SAA3) by bovine mammary epithelial cells following stimulation with either prolactin (PRL) or lipopolysaccharide (LPS). Reverse transcription-polymerase chain reaction analysis of PRL or LPS induced bovine mammary epithelial cells resulted in the detection of only the mammary-derived Saa3 (M-Saa3) transcript. Two-dimensional immunoblot analyses of colostrum and milk from healthy cows, as well as conditioned medium from PRL or LPS stimulated bovine mammary epithelial cells confirmed the differential production and secretion of M-SAA3 while other SAA isoforms were not detected. These data indicate that the bovine Saa3 gene is regulated differently from the other Saa genes with regard to the site of and stimulus for expression, suggesting an important tissue-specific function for bovine M-SAA3 during lactation and mammary infection.

AB - Serum amyloid A (SAA) proteins were originally identified as prominent acute phase serum reactants synthesized predominately by hepatocytes in response to infection, inflammation and trauma. In this study, we report the differential expression and secretion of serum amyloid A3 (SAA3) by bovine mammary epithelial cells following stimulation with either prolactin (PRL) or lipopolysaccharide (LPS). Reverse transcription-polymerase chain reaction analysis of PRL or LPS induced bovine mammary epithelial cells resulted in the detection of only the mammary-derived Saa3 (M-Saa3) transcript. Two-dimensional immunoblot analyses of colostrum and milk from healthy cows, as well as conditioned medium from PRL or LPS stimulated bovine mammary epithelial cells confirmed the differential production and secretion of M-SAA3 while other SAA isoforms were not detected. These data indicate that the bovine Saa3 gene is regulated differently from the other Saa genes with regard to the site of and stimulus for expression, suggesting an important tissue-specific function for bovine M-SAA3 during lactation and mammary infection.

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KW - Prolactin

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