Differential effects of grape powder and its extract on glucose tolerance and chronic inflammation in high-fat-fed obese mice

Chia Chi Chuang, Wan Shen, Huiyuan Chen, Guoxiang Xie, Wei Jia, Soonkyu Chung, Michael K. McIntosh

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The objective of this study was to determine the anti-inflammatory properties of grape powder (GP) or GP extract (GE) and examine (1) which polyphenol metabolites in GE were bioavailable, (2) the impact of GP and GE on glucose tolerance and inflammation in obese mice, and (3) if bioavailable polyphenols in GE decrease markers of inflammation in primary adipocytes. In experiment 1, C57BL/6J mice were gavaged with GE and serum polyphenols were measured. In experiment 2, mice were fed high-fat diets supplemented with 3% GP or 0.02% GE for 18 weeks and markers of inflammation were measured. In experiment 3, human adipocytes were treated with the bioavailable polyphenols quercetin 3-O-glucoside (Q3G) or quercetin 3-O-glucuronide (Q3GN) and markers of inflammation were measured. Serum Q3G and Q3GN increased at 1 h post-GE gavage and decreased thereafter. GP supplementation improved glucose tolerance at 5 weeks and decreased markers of inflammation ∼20-50% in serum and adipose tissue at 18 weeks. Q3G, but not Q3GN, attenuated TNFα-mediated inflammatory gene expression ∼30-40% in human adipocytes, possibly by suppressing c-Jun-NH2 terminal kinase and c-Jun activation. In summary, (1) Q3G and Q3GN are bioavailable polyphenols in GE, (2) GP acutely improves glucose tolerance and chronically reduces markers of inflammation in obese mice, and (3) Q3G reduces several markers of inflammation in human adipocytes.

Original languageEnglish (US)
Pages (from-to)12458-12468
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number51
DOIs
StatePublished - Dec 26 2012

Fingerprint

Obese Mice
Vitis
glucose tolerance
quercetin
Powders
Quercetin
Polyphenols
grapes
powders
inflammation
Fats
Inflammation
Glucose
Adipocytes
mice
Glucosides
extracts
lipids
glucosides
polyphenols

Keywords

  • glucose tolerance
  • grape powder
  • grape powder extract
  • inflammation
  • quercetin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Differential effects of grape powder and its extract on glucose tolerance and chronic inflammation in high-fat-fed obese mice. / Chuang, Chia Chi; Shen, Wan; Chen, Huiyuan; Xie, Guoxiang; Jia, Wei; Chung, Soonkyu; McIntosh, Michael K.

In: Journal of Agricultural and Food Chemistry, Vol. 60, No. 51, 26.12.2012, p. 12458-12468.

Research output: Contribution to journalArticle

Chuang, Chia Chi ; Shen, Wan ; Chen, Huiyuan ; Xie, Guoxiang ; Jia, Wei ; Chung, Soonkyu ; McIntosh, Michael K. / Differential effects of grape powder and its extract on glucose tolerance and chronic inflammation in high-fat-fed obese mice. In: Journal of Agricultural and Food Chemistry. 2012 ; Vol. 60, No. 51. pp. 12458-12468.
@article{9261ddfd5b554752bea50c903ae40ec1,
title = "Differential effects of grape powder and its extract on glucose tolerance and chronic inflammation in high-fat-fed obese mice",
abstract = "The objective of this study was to determine the anti-inflammatory properties of grape powder (GP) or GP extract (GE) and examine (1) which polyphenol metabolites in GE were bioavailable, (2) the impact of GP and GE on glucose tolerance and inflammation in obese mice, and (3) if bioavailable polyphenols in GE decrease markers of inflammation in primary adipocytes. In experiment 1, C57BL/6J mice were gavaged with GE and serum polyphenols were measured. In experiment 2, mice were fed high-fat diets supplemented with 3{\%} GP or 0.02{\%} GE for 18 weeks and markers of inflammation were measured. In experiment 3, human adipocytes were treated with the bioavailable polyphenols quercetin 3-O-glucoside (Q3G) or quercetin 3-O-glucuronide (Q3GN) and markers of inflammation were measured. Serum Q3G and Q3GN increased at 1 h post-GE gavage and decreased thereafter. GP supplementation improved glucose tolerance at 5 weeks and decreased markers of inflammation ∼20-50{\%} in serum and adipose tissue at 18 weeks. Q3G, but not Q3GN, attenuated TNFα-mediated inflammatory gene expression ∼30-40{\%} in human adipocytes, possibly by suppressing c-Jun-NH2 terminal kinase and c-Jun activation. In summary, (1) Q3G and Q3GN are bioavailable polyphenols in GE, (2) GP acutely improves glucose tolerance and chronically reduces markers of inflammation in obese mice, and (3) Q3G reduces several markers of inflammation in human adipocytes.",
keywords = "glucose tolerance, grape powder, grape powder extract, inflammation, quercetin",
author = "Chuang, {Chia Chi} and Wan Shen and Huiyuan Chen and Guoxiang Xie and Wei Jia and Soonkyu Chung and McIntosh, {Michael K.}",
year = "2012",
month = "12",
day = "26",
doi = "10.1021/jf3028107",
language = "English (US)",
volume = "60",
pages = "12458--12468",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "51",

}

TY - JOUR

T1 - Differential effects of grape powder and its extract on glucose tolerance and chronic inflammation in high-fat-fed obese mice

AU - Chuang, Chia Chi

AU - Shen, Wan

AU - Chen, Huiyuan

AU - Xie, Guoxiang

AU - Jia, Wei

AU - Chung, Soonkyu

AU - McIntosh, Michael K.

PY - 2012/12/26

Y1 - 2012/12/26

N2 - The objective of this study was to determine the anti-inflammatory properties of grape powder (GP) or GP extract (GE) and examine (1) which polyphenol metabolites in GE were bioavailable, (2) the impact of GP and GE on glucose tolerance and inflammation in obese mice, and (3) if bioavailable polyphenols in GE decrease markers of inflammation in primary adipocytes. In experiment 1, C57BL/6J mice were gavaged with GE and serum polyphenols were measured. In experiment 2, mice were fed high-fat diets supplemented with 3% GP or 0.02% GE for 18 weeks and markers of inflammation were measured. In experiment 3, human adipocytes were treated with the bioavailable polyphenols quercetin 3-O-glucoside (Q3G) or quercetin 3-O-glucuronide (Q3GN) and markers of inflammation were measured. Serum Q3G and Q3GN increased at 1 h post-GE gavage and decreased thereafter. GP supplementation improved glucose tolerance at 5 weeks and decreased markers of inflammation ∼20-50% in serum and adipose tissue at 18 weeks. Q3G, but not Q3GN, attenuated TNFα-mediated inflammatory gene expression ∼30-40% in human adipocytes, possibly by suppressing c-Jun-NH2 terminal kinase and c-Jun activation. In summary, (1) Q3G and Q3GN are bioavailable polyphenols in GE, (2) GP acutely improves glucose tolerance and chronically reduces markers of inflammation in obese mice, and (3) Q3G reduces several markers of inflammation in human adipocytes.

AB - The objective of this study was to determine the anti-inflammatory properties of grape powder (GP) or GP extract (GE) and examine (1) which polyphenol metabolites in GE were bioavailable, (2) the impact of GP and GE on glucose tolerance and inflammation in obese mice, and (3) if bioavailable polyphenols in GE decrease markers of inflammation in primary adipocytes. In experiment 1, C57BL/6J mice were gavaged with GE and serum polyphenols were measured. In experiment 2, mice were fed high-fat diets supplemented with 3% GP or 0.02% GE for 18 weeks and markers of inflammation were measured. In experiment 3, human adipocytes were treated with the bioavailable polyphenols quercetin 3-O-glucoside (Q3G) or quercetin 3-O-glucuronide (Q3GN) and markers of inflammation were measured. Serum Q3G and Q3GN increased at 1 h post-GE gavage and decreased thereafter. GP supplementation improved glucose tolerance at 5 weeks and decreased markers of inflammation ∼20-50% in serum and adipose tissue at 18 weeks. Q3G, but not Q3GN, attenuated TNFα-mediated inflammatory gene expression ∼30-40% in human adipocytes, possibly by suppressing c-Jun-NH2 terminal kinase and c-Jun activation. In summary, (1) Q3G and Q3GN are bioavailable polyphenols in GE, (2) GP acutely improves glucose tolerance and chronically reduces markers of inflammation in obese mice, and (3) Q3G reduces several markers of inflammation in human adipocytes.

KW - glucose tolerance

KW - grape powder

KW - grape powder extract

KW - inflammation

KW - quercetin

UR - http://www.scopus.com/inward/record.url?scp=84871602044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871602044&partnerID=8YFLogxK

U2 - 10.1021/jf3028107

DO - 10.1021/jf3028107

M3 - Article

C2 - 23210691

AN - SCOPUS:84871602044

VL - 60

SP - 12458

EP - 12468

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 51

ER -