Differential cellular susceptibility to Epstein‐Barr virus infection in a patient with X‐linked lymphoproliferative disease

M. Okano, Geoffrey Milton Thiele, T. G. Gross, J. R. Davis, D. T. Purtilo

Research output: Contribution to journalArticle

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Abstract

Epstein‐Barr virus (EBV) is often associated with lethal lymphoproliferative disease in immunologically compromised individuals. Recently, we have studied a 20‐month‐old boy with X‐linked lymphoproliferative disease (XLP) who had succumbed to infectious mononucleosis (IM) complicated by fulminant hepatitis and virus‐associated hemophagocytic syndrome following EBV infection. EBV genomes were detected in peripheral blood lymphocytes (PBL), cervical and mesenteric lymph nodes, liver, spleen, thymus, and bone marrow. According to restriction endonuclease analyses, the EBV‐DNA pattern was similar in all samples except for the EBV‐DNA from the bone marrow. Additionally, circular EBV‐DNA (suggesting a latent infection) predominated in spontaneously established lymphoblastoid cell lines (LCLs) derived from both the lymph node and cord lymphocytes co‐cultured with PBL. In contrast, both circular and linear EBV‐DNA (suggesting a lytic infection) were noted in spontaneously established LCLs derived from his PBL. Furthermore, LCLs derived from both the lymph node and cord lymphocytes co‐cultured with PBL expressed fewer reactive cells for early antigen (EA) and viral capsid antigen (VCA) than spontaneous LCLs from his PBL, thus providing evidence for different B cellular susceptibility to EBV infection in this patient with XLP. Finally, defective EBV‐specific cytotoxic T cell activity was observed in this patient. Latent EBV infected cells may easily escape immuno‐surveillance by the host. These findings may explain the fatal course of EBV infection in this patient.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalJournal of Medical Virology
Volume32
Issue number1
DOIs
StatePublished - Sep 1990

Fingerprint

Virus Diseases
Lymphocytes
Cell Line
Lymph Nodes
Viruses
Bone Marrow
Hemophagocytic Lymphohistiocytosis
Immunologic Monitoring
Infectious Mononucleosis
Viral Antigens
Capsid
DNA Restriction Enzymes
Infection
Thymus Gland
Hepatitis
Spleen
Genome
T-Lymphocytes
Antigens
Liver

Keywords

  • endonuclease
  • lymphoblastoid
  • lymphocyte

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Differential cellular susceptibility to Epstein‐Barr virus infection in a patient with X‐linked lymphoproliferative disease. / Okano, M.; Thiele, Geoffrey Milton; Gross, T. G.; Davis, J. R.; Purtilo, D. T.

In: Journal of Medical Virology, Vol. 32, No. 1, 09.1990, p. 47-52.

Research output: Contribution to journalArticle

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