Differential activation of the tyrosine kinases ZAP-70 and Syk after FcγRI stimulation

Naomi Taylor, Thomas Jahn, Susan Smith, Thomas Lamkin, Lisa Uribe, Yenbou Liu, Donald L. Durden, Kenneth Weinberg

Research output: Contribution to journalArticle

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Abstract

Engagement of the high-affinity IgG Fc receptor (FcγRI) activates a signal transduction pathway involving tyrosine phosphorylation of associated kinases. We compared the activation of the related protein tyrosine kinases (PTKs), Syk and ZAP-70, in FcγRI-mediated signaling. Cross-linking of the FcγRI multimeric receptor in monocytic cells results in tyrosine phosphorylation of the FcεRIγ subunit and association of Syk with this complex. We stably introduced ZAP-70 via a retroviral vector into two monocytic cell lines, U937 and THP-1, which normally do not express ZAP-70. Neither Syk nor MAP kinase activation was affected by the presence of ZAP- 70. Although transduced ZAP-70 had in vitro kinase activity and associated with FcεRIγ after receptor aggregation, it was not tyrosine phosphorylated. In contrast, both ZAP-70 and Syk were phosphorylated in a T-cell line in which their respective levels of expression were similar to those detected in U937/ZAP-70 cells. Therefore, these results suggest that requirements for Syk and ZAP-70 phosphorylation are distinct in a monocytic cell context.

Original languageEnglish (US)
Pages (from-to)388-396
Number of pages9
JournalBlood
Volume89
Issue number2
StatePublished - Jan 15 1997

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Phosphorylation
Protein-Tyrosine Kinases
Tyrosine
Phosphotransferases
Chemical activation
ZAP-70 Protein-Tyrosine Kinase
Receptor Aggregation
IgG Receptors
Cell Line
Signal transduction
T-cells
Fc Receptors
Signal Transduction
Agglomeration
Immunoglobulin G
Cells
Association reactions
T-Lymphocytes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Taylor, N., Jahn, T., Smith, S., Lamkin, T., Uribe, L., Liu, Y., ... Weinberg, K. (1997). Differential activation of the tyrosine kinases ZAP-70 and Syk after FcγRI stimulation. Blood, 89(2), 388-396.

Differential activation of the tyrosine kinases ZAP-70 and Syk after FcγRI stimulation. / Taylor, Naomi; Jahn, Thomas; Smith, Susan; Lamkin, Thomas; Uribe, Lisa; Liu, Yenbou; Durden, Donald L.; Weinberg, Kenneth.

In: Blood, Vol. 89, No. 2, 15.01.1997, p. 388-396.

Research output: Contribution to journalArticle

Taylor, N, Jahn, T, Smith, S, Lamkin, T, Uribe, L, Liu, Y, Durden, DL & Weinberg, K 1997, 'Differential activation of the tyrosine kinases ZAP-70 and Syk after FcγRI stimulation', Blood, vol. 89, no. 2, pp. 388-396.
Taylor N, Jahn T, Smith S, Lamkin T, Uribe L, Liu Y et al. Differential activation of the tyrosine kinases ZAP-70 and Syk after FcγRI stimulation. Blood. 1997 Jan 15;89(2):388-396.
Taylor, Naomi ; Jahn, Thomas ; Smith, Susan ; Lamkin, Thomas ; Uribe, Lisa ; Liu, Yenbou ; Durden, Donald L. ; Weinberg, Kenneth. / Differential activation of the tyrosine kinases ZAP-70 and Syk after FcγRI stimulation. In: Blood. 1997 ; Vol. 89, No. 2. pp. 388-396.
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