Different epidermal growth factor growth responses and receptor levels in human colon carcinoma cell lines

C. W. Wan, M. K. McKnight, D. E. Brattain, M. G. Brattain, L. C. Yeoman

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The growth response to epidermal growth factor (EGF) and the numbers and types of EGF receptors were studied in three human colon tumor cell lines from each of two groups of cell lines that differ markedly in their growth properties and extent of differentiation. Aggressively growing and poorly differentiated colon cells (group I) did not respond to EGF alone, while less aggressively growing and more differentiated cells (group III) responded with increased growth when EGF was added to their chemically defined, serum-free medium. The average number of EGF receptors (EGF-R) measured at the surface of group III cell lines by radioligand binding assays, was eight-fold higher than that measured for group I cell lines. These observations provide evidence for possible autocrine mechanisms that maintain available EGF-R levels in more differentiated group III colon tumor cells and down-regulate EGF-R levels in group I colon tumor cells.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalCancer Letters
Volume43
Issue number1-2
DOIs
StatePublished - Dec 1 1988

Fingerprint

Epidermal Growth Factor Receptor
Epidermal Growth Factor
Colon
Carcinoma
Cell Line
Growth
Radioligand Assay
Serum-Free Culture Media
Tumor Cell Line
Neoplasms
Down-Regulation
ErbB Receptors

Keywords

  • colon
  • differentiation
  • epidermal growth factor
  • growth
  • growth factor
  • receptor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Different epidermal growth factor growth responses and receptor levels in human colon carcinoma cell lines. / Wan, C. W.; McKnight, M. K.; Brattain, D. E.; Brattain, M. G.; Yeoman, L. C.

In: Cancer Letters, Vol. 43, No. 1-2, 01.12.1988, p. 139-143.

Research output: Contribution to journalArticle

Wan, C. W. ; McKnight, M. K. ; Brattain, D. E. ; Brattain, M. G. ; Yeoman, L. C. / Different epidermal growth factor growth responses and receptor levels in human colon carcinoma cell lines. In: Cancer Letters. 1988 ; Vol. 43, No. 1-2. pp. 139-143.
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