Differences among antimicrobial properties of carrion beetle secretions reflect phylogeny and ecology

W. Wyatt Hoback, Andrew A. Bishop, Jeremy Kroemer, Joanne Scalzitti, Julie J Shaffer

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Carrion beetles (Coleoptera: Silphidae) consist of two subfamilies in North America. Members of the Silphinae arrive at carcasses during the mid-stage of decay and their larvae feed on developing maggots, while members of the Nicrophorinae bury and tend carcasses upon which their developing larvae feed. The Nicrophorinae maintain the condition of the carcass by applying oral and anal secretions that reduce carcass decay apparently through bacterial inhibition, although quantification has not been made. We hypothesized that enzymes in the oral and anal secretions of the subfamily Nicrophorinae would inhibit bacterial growth, while secretions from the subfamily Silphinae would not. The secretions were assayed for inhibitory effects with a Microtox Analyzer that monitors the decrease in bioluminescence from the bacterium Vibrio fischerii. We found a significant difference of bioluminescence in the control compared to secretions of 8 out of 10 tested Nicrophorinae (with oral secretions being most active), while only anal secretions from Necrodes surinimensis of the Siphinae significantly reduced bacterial survival. These data follow the known phylogenic relationship in which Necrodes is the closest genus to the Nicrophorinae. The two species of Nicrophorinae, which did not show significant reductions in bacterial growth, differ ecologically from the others. Thus, the presence of antimicrobial compounds in most Nicrophorinae secretions, but not in most other Silphinae, represents an adaptation to preserve the buried carcass.

Original languageEnglish (US)
Pages (from-to)719-729
Number of pages11
JournalJournal of Chemical Ecology
Volume30
Issue number4
DOIs
StatePublished - Apr 1 2004

Fingerprint

Silphidae
Bioluminescence
carrion
Beetles
anti-infective properties
Ecology
Phylogeny
secretion
Larva
beetle
phylogeny
ecology
Bacteria
Vibrio
Growth
North America
mouth
bioluminescence
Enzymes
microbial growth

Keywords

  • Antimicrobial
  • Burying beetle
  • Carrion beetle
  • Nicrophorus
  • Silphidae

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Differences among antimicrobial properties of carrion beetle secretions reflect phylogeny and ecology. / Hoback, W. Wyatt; Bishop, Andrew A.; Kroemer, Jeremy; Scalzitti, Joanne; Shaffer, Julie J.

In: Journal of Chemical Ecology, Vol. 30, No. 4, 01.04.2004, p. 719-729.

Research output: Contribution to journalArticle

Hoback, W. Wyatt ; Bishop, Andrew A. ; Kroemer, Jeremy ; Scalzitti, Joanne ; Shaffer, Julie J. / Differences among antimicrobial properties of carrion beetle secretions reflect phylogeny and ecology. In: Journal of Chemical Ecology. 2004 ; Vol. 30, No. 4. pp. 719-729.
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