Dietary supplementation with (R)-α-lipoic acid reverses the age-related accumulation of iron and depletion of antioxidants in the rat cerebral cortex

Jung H. Suh, Régis Moreau, Shi Hua D. Heath, Tory M. Hagen

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Accumulation of divalent metal ions (e.g. iron and copper) has been proposed to contribute to heightened oxidative stress evident in aging and neurodegenerative disorders. To understand the extent of iron accumulation and its effect on antioxidant status, we monitored iron content in the cerebral cortex of F344 rats by inductively coupled plasma atomic emission spectrometry (ICP-AES) and found that the cerebral iron levels in 24-28-month-old rats were increased by 80% (p<0.01) relative to 3-month-old rats. Iron accumulation correlated with a decline in glutathione (GSH) and the GSH/GSSG ratio, indicating that iron accumulation altered antioxidant capacity and thiol redox state in aged animals. Because (A)-α-Lipoic acid (LA) is a potent chelator of divalent metal ions in vitro and also regenerates other antioxidants, we monitored whether feeding LA (0.2% [w/w]; 2 weeks) could lower cortical iron and improve antioxidant status. Results show that cerebral iron levels in old LA-fed animals were lower when compared to controls and were similar to levels seen in young rats. Antioxidant status and thiol redox state also improved markedly in old LA-fed rats versus controls. These results thus show that LA supplementation may be a means to modulate the age-related accumulation of cortical iron content, thereby lowering oxidative stress associated with aging.

Original languageEnglish (US)
Pages (from-to)52-60
Number of pages9
JournalRedox Report
Volume10
Issue number1
DOIs
StatePublished - Feb 1 2005

Fingerprint

Thioctic Acid
Dietary Supplements
Cerebral Cortex
Rats
Iron
Antioxidants
Oxidative stress
Sulfhydryl Compounds
Oxidation-Reduction
Metal ions
Animals
Oxidative Stress
Aging of materials
Metals
Rat control
Ions
Glutathione Disulfide
Inbred F344 Rats
Inductively coupled plasma
Chelating Agents

Keywords

  • (R)-α-lipoic acid
  • Antioxidant depletion
  • Chronic oxidative stress
  • Iron accumulation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical

Cite this

Dietary supplementation with (R)-α-lipoic acid reverses the age-related accumulation of iron and depletion of antioxidants in the rat cerebral cortex. / Suh, Jung H.; Moreau, Régis; Heath, Shi Hua D.; Hagen, Tory M.

In: Redox Report, Vol. 10, No. 1, 01.02.2005, p. 52-60.

Research output: Contribution to journalArticle

@article{58af128ffd6645f9a39cf42c0da25143,
title = "Dietary supplementation with (R)-α-lipoic acid reverses the age-related accumulation of iron and depletion of antioxidants in the rat cerebral cortex",
abstract = "Accumulation of divalent metal ions (e.g. iron and copper) has been proposed to contribute to heightened oxidative stress evident in aging and neurodegenerative disorders. To understand the extent of iron accumulation and its effect on antioxidant status, we monitored iron content in the cerebral cortex of F344 rats by inductively coupled plasma atomic emission spectrometry (ICP-AES) and found that the cerebral iron levels in 24-28-month-old rats were increased by 80{\%} (p<0.01) relative to 3-month-old rats. Iron accumulation correlated with a decline in glutathione (GSH) and the GSH/GSSG ratio, indicating that iron accumulation altered antioxidant capacity and thiol redox state in aged animals. Because (A)-α-Lipoic acid (LA) is a potent chelator of divalent metal ions in vitro and also regenerates other antioxidants, we monitored whether feeding LA (0.2{\%} [w/w]; 2 weeks) could lower cortical iron and improve antioxidant status. Results show that cerebral iron levels in old LA-fed animals were lower when compared to controls and were similar to levels seen in young rats. Antioxidant status and thiol redox state also improved markedly in old LA-fed rats versus controls. These results thus show that LA supplementation may be a means to modulate the age-related accumulation of cortical iron content, thereby lowering oxidative stress associated with aging.",
keywords = "(R)-α-lipoic acid, Antioxidant depletion, Chronic oxidative stress, Iron accumulation",
author = "Suh, {Jung H.} and R{\'e}gis Moreau and Heath, {Shi Hua D.} and Hagen, {Tory M.}",
year = "2005",
month = "2",
day = "1",
doi = "10.1179/135100005X21624",
language = "English (US)",
volume = "10",
pages = "52--60",
journal = "Redox Report",
issn = "1351-0002",
publisher = "Maney Publishing",
number = "1",

}

TY - JOUR

T1 - Dietary supplementation with (R)-α-lipoic acid reverses the age-related accumulation of iron and depletion of antioxidants in the rat cerebral cortex

AU - Suh, Jung H.

AU - Moreau, Régis

AU - Heath, Shi Hua D.

AU - Hagen, Tory M.

PY - 2005/2/1

Y1 - 2005/2/1

N2 - Accumulation of divalent metal ions (e.g. iron and copper) has been proposed to contribute to heightened oxidative stress evident in aging and neurodegenerative disorders. To understand the extent of iron accumulation and its effect on antioxidant status, we monitored iron content in the cerebral cortex of F344 rats by inductively coupled plasma atomic emission spectrometry (ICP-AES) and found that the cerebral iron levels in 24-28-month-old rats were increased by 80% (p<0.01) relative to 3-month-old rats. Iron accumulation correlated with a decline in glutathione (GSH) and the GSH/GSSG ratio, indicating that iron accumulation altered antioxidant capacity and thiol redox state in aged animals. Because (A)-α-Lipoic acid (LA) is a potent chelator of divalent metal ions in vitro and also regenerates other antioxidants, we monitored whether feeding LA (0.2% [w/w]; 2 weeks) could lower cortical iron and improve antioxidant status. Results show that cerebral iron levels in old LA-fed animals were lower when compared to controls and were similar to levels seen in young rats. Antioxidant status and thiol redox state also improved markedly in old LA-fed rats versus controls. These results thus show that LA supplementation may be a means to modulate the age-related accumulation of cortical iron content, thereby lowering oxidative stress associated with aging.

AB - Accumulation of divalent metal ions (e.g. iron and copper) has been proposed to contribute to heightened oxidative stress evident in aging and neurodegenerative disorders. To understand the extent of iron accumulation and its effect on antioxidant status, we monitored iron content in the cerebral cortex of F344 rats by inductively coupled plasma atomic emission spectrometry (ICP-AES) and found that the cerebral iron levels in 24-28-month-old rats were increased by 80% (p<0.01) relative to 3-month-old rats. Iron accumulation correlated with a decline in glutathione (GSH) and the GSH/GSSG ratio, indicating that iron accumulation altered antioxidant capacity and thiol redox state in aged animals. Because (A)-α-Lipoic acid (LA) is a potent chelator of divalent metal ions in vitro and also regenerates other antioxidants, we monitored whether feeding LA (0.2% [w/w]; 2 weeks) could lower cortical iron and improve antioxidant status. Results show that cerebral iron levels in old LA-fed animals were lower when compared to controls and were similar to levels seen in young rats. Antioxidant status and thiol redox state also improved markedly in old LA-fed rats versus controls. These results thus show that LA supplementation may be a means to modulate the age-related accumulation of cortical iron content, thereby lowering oxidative stress associated with aging.

KW - (R)-α-lipoic acid

KW - Antioxidant depletion

KW - Chronic oxidative stress

KW - Iron accumulation

UR - http://www.scopus.com/inward/record.url?scp=27644516440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27644516440&partnerID=8YFLogxK

U2 - 10.1179/135100005X21624

DO - 10.1179/135100005X21624

M3 - Article

C2 - 15829111

AN - SCOPUS:27644516440

VL - 10

SP - 52

EP - 60

JO - Redox Report

JF - Redox Report

SN - 1351-0002

IS - 1

ER -