Dichlorvos, chlorpyrifos oxon and Aldicarb adducts of butyrylcholinesterase, detected by mass spectrometry in human plasma following deliberate overdose

Bin Li, Ivan Ricordel, Lawrence M Schopfer, Frédéric Baud, Bruno Mégarbane, Patrick Massond, Oksana Lockridgea

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The goal of this study was to develop a method to detect pesticide adducts in tryptic digests of butyrylcholinesterase in human plasma from patients poisoned by pesticides. Adducts to butyrylcholinesterase in human serum may serve as biomarkers of pesticide exposure because organophosphorus and carbamate pesticides make a covalent bond with the active site serine of butyrylcholinesterase. Serum samples from five attempted suicides (with dichlorvos, Aldicarb, Baygon and an unknown pesticide) and from one patient who accidentally inhaled dichlorvos were analyzed. Butyrylcholinesterase was purified from 2 ml serum by ion exchange chromatography at pH 4, followed by procainamide affinity chromatography at pH 7. The purified butyrylcholinesterase was denatured, digested with trypsin and the modified peptide isolated by HPLC. The purified peptide was analyzed by multiple reaction monitoring in a QTRAP 4000 mass spectrometer. This method successfully identified the pesticide-adducted butyrylcholinesterase peptide in four patients whose butyrylcholinesterase was inhibited 60-84%, but not in two patients whose inhibition levels were 8 and 22%. It is expected that low inhibition levels will require analysis of larger serum plasma volumes. In conclusion, a mass spectrometry method for identification of exposure to live toxic pesticides has been developed, based on identification of pesticide adducts on the active site serine of human butyrylcholinesterase.

Original languageEnglish (US)
Pages (from-to)559-565
Number of pages7
JournalJournal of Applied Toxicology
Volume30
Issue number6
DOIs
StatePublished - Aug 1 2010

Fingerprint

Aldicarb
Dichlorvos
Plasma (human)
Butyrylcholinesterase
Pesticides
Mass spectrometry
Mass Spectrometry
Serum
Serine
Peptides
Catalytic Domain
Propoxur
Affinity chromatography
Procainamide
Attempted Suicide
Covalent bonds
Carbamates
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate
Plasma Volume
Poisons

Keywords

  • Aldicarb
  • Butyrylcholinesterase
  • Chlorpyrifos oxon
  • Dichlorvos
  • Mass spectrometry
  • Pesticide poisoning biomarker

ASJC Scopus subject areas

  • Toxicology

Cite this

Dichlorvos, chlorpyrifos oxon and Aldicarb adducts of butyrylcholinesterase, detected by mass spectrometry in human plasma following deliberate overdose. / Li, Bin; Ricordel, Ivan; Schopfer, Lawrence M; Baud, Frédéric; Mégarbane, Bruno; Massond, Patrick; Lockridgea, Oksana.

In: Journal of Applied Toxicology, Vol. 30, No. 6, 01.08.2010, p. 559-565.

Research output: Contribution to journalArticle

Li, Bin ; Ricordel, Ivan ; Schopfer, Lawrence M ; Baud, Frédéric ; Mégarbane, Bruno ; Massond, Patrick ; Lockridgea, Oksana. / Dichlorvos, chlorpyrifos oxon and Aldicarb adducts of butyrylcholinesterase, detected by mass spectrometry in human plasma following deliberate overdose. In: Journal of Applied Toxicology. 2010 ; Vol. 30, No. 6. pp. 559-565.
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