Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells

Rocio Rodriguez-Melendez, Brandon Lewis, Robert J. McMahon, Janos Zempleni

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)γ also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues are quantitatively important. Here, the hypothesis was tested that biotin metabolites can mimic the effects of biotin on gene expression and thus have biotin-like activities. A human T-cell line (Jurkat cells) was used to model effects of biotin and synthetic metabolites (diaminobiotin and desthiobiotin) on the expression of genes encoding IL-2 and IL-2Rγ. Cells were cultured in biotin-deficient medium (0.025 nmol/L biotin) for 35 d; controls were cultured in medium containing 10 nmol/L biotin. The biotin-deficient medium was supplemented with 10 nmol/L of diaminobiotin, desthiobiotin, biotin or no biotin 24 h before gene expression analyses. Transcriptional activities of genes encoding IL-2 and IL-2Rγ were increased up to 43% in cells supplemented with diaminobiotin, desthiobiotin or biotin compared with biotindeficient cells, as judged by luciferase activities after transfection with reporter-gene constructs. These findings are consistent with the hypothesis that diaminobiotin and desthiobiotin mimic the effects of biotin on gene expression and thus have biotin-like activities. Supplementation of cells with diaminobiotin and desthiobiotin did not affect abundances of holocarboxylases and activities of propionyl-CoA carboxylase, suggesting that effects of synthetic biotin metabolites on gene expression are not mediated by carboxylase-dependent pathways. It is not known whether naturally occurring biotin metabolites also have biotin-like activities.

Original languageEnglish (US)
Pages (from-to)1259-1264
Number of pages6
JournalJournal of Nutrition
Volume133
Issue number5
StatePublished - May 1 2003

Fingerprint

Jurkat Cells
biotin
Biotin
cells
interleukin-2
Gene Expression
gene expression
metabolites
Interleukin-2 Receptors
propionyl-CoA carboxylase
Methylmalonyl-CoA Decarboxylase
diaminobiotin
desthiobiotin
Interleukin-2
receptors

Keywords

  • Biotin metabolites
  • Gene expression
  • Humans
  • Interleukin-2
  • Jurkat cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Rodriguez-Melendez, R., Lewis, B., McMahon, R. J., & Zempleni, J. (2003). Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells. Journal of Nutrition, 133(5), 1259-1264.

Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells. / Rodriguez-Melendez, Rocio; Lewis, Brandon; McMahon, Robert J.; Zempleni, Janos.

In: Journal of Nutrition, Vol. 133, No. 5, 01.05.2003, p. 1259-1264.

Research output: Contribution to journalArticle

Rodriguez-Melendez, R, Lewis, B, McMahon, RJ & Zempleni, J 2003, 'Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells', Journal of Nutrition, vol. 133, no. 5, pp. 1259-1264.
Rodriguez-Melendez R, Lewis B, McMahon RJ, Zempleni J. Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells. Journal of Nutrition. 2003 May 1;133(5):1259-1264.
Rodriguez-Melendez, Rocio ; Lewis, Brandon ; McMahon, Robert J. ; Zempleni, Janos. / Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells. In: Journal of Nutrition. 2003 ; Vol. 133, No. 5. pp. 1259-1264.
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abstract = "In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)γ also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues are quantitatively important. Here, the hypothesis was tested that biotin metabolites can mimic the effects of biotin on gene expression and thus have biotin-like activities. A human T-cell line (Jurkat cells) was used to model effects of biotin and synthetic metabolites (diaminobiotin and desthiobiotin) on the expression of genes encoding IL-2 and IL-2Rγ. Cells were cultured in biotin-deficient medium (0.025 nmol/L biotin) for 35 d; controls were cultured in medium containing 10 nmol/L biotin. The biotin-deficient medium was supplemented with 10 nmol/L of diaminobiotin, desthiobiotin, biotin or no biotin 24 h before gene expression analyses. Transcriptional activities of genes encoding IL-2 and IL-2Rγ were increased up to 43{\%} in cells supplemented with diaminobiotin, desthiobiotin or biotin compared with biotindeficient cells, as judged by luciferase activities after transfection with reporter-gene constructs. These findings are consistent with the hypothesis that diaminobiotin and desthiobiotin mimic the effects of biotin on gene expression and thus have biotin-like activities. Supplementation of cells with diaminobiotin and desthiobiotin did not affect abundances of holocarboxylases and activities of propionyl-CoA carboxylase, suggesting that effects of synthetic biotin metabolites on gene expression are not mediated by carboxylase-dependent pathways. It is not known whether naturally occurring biotin metabolites also have biotin-like activities.",
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