Diallyl sulfide inhibits diethylstilbestrol induced DNA damage in human breast epithelial cells (MCF-10A)

Michael L. McCaskill, Eleanor G Rogan, Ronald D. Thomas

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Breast cancer is the second leading cause of cancer deaths in women in the United States. Diethylstilbestrol (DES) is a synthetic estrogen that has been shown to cause cancer in animals and humans, altering cell viability as well as inducing DNA damage. Diallyl sulfide (DAS) is a garlic organosulfide that has been shown to inhibit both the initiation and promotion phases of cancer in vivo and in vitro, as well as reduce the risk of cancer in epidemiological studies. MCF-10A cells, regarded as a normal breast epithelial cell line, were treated with varying concentrations of DES, DAS or various dose combinations of DES and DAS concomitantly, and assessed for cell viability, DNA strand breaks, and lipid peroxidation. DES (10 μM) in combination with 1, 10, or 100 μM DAS resulted in a 31%, 34%, or 36% respective increase in cell viability compared to the DES treatment alone, after 24 h. At the same time point, 1, 10, and 100 μM DAS were all effective in significantly reducing DES (100 μM)-induced strand breaks to near that of the vehicle control. Additionally, 1 μM DAS was effective in significantly reducing DES (100 μM)-induced lipid peroxidation after 3 h. The results of this research suggest that DAS is effective in recovering cell viability, attenuating DNA strand breaks, and decreasing lipid peroxidation in MCF-10A cells.

Original languageEnglish (US)
Pages (from-to)96-100
Number of pages5
JournalSteroids
Volume92
DOIs
StatePublished - Dec 15 2014

Fingerprint

Diethylstilbestrol
DNA Damage
Breast
Epithelial Cells
DNA
Cell Survival
Cells
Lipid Peroxidation
DNA Breaks
Lipids
Neoplasms
Estradiol Congeners
Garlic
allyl sulfide
Epidemiologic Studies
Cause of Death
Animals
Breast Neoplasms
Cell Line
Research

Keywords

  • Breast epithelial cells chemoprevention
  • DNA damage
  • Diallyl sulfide
  • Diethystilbestrol

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Diallyl sulfide inhibits diethylstilbestrol induced DNA damage in human breast epithelial cells (MCF-10A). / McCaskill, Michael L.; Rogan, Eleanor G; Thomas, Ronald D.

In: Steroids, Vol. 92, 15.12.2014, p. 96-100.

Research output: Contribution to journalArticle

@article{f76e194a633347a5b48dc22bfe47f98f,
title = "Diallyl sulfide inhibits diethylstilbestrol induced DNA damage in human breast epithelial cells (MCF-10A)",
abstract = "Breast cancer is the second leading cause of cancer deaths in women in the United States. Diethylstilbestrol (DES) is a synthetic estrogen that has been shown to cause cancer in animals and humans, altering cell viability as well as inducing DNA damage. Diallyl sulfide (DAS) is a garlic organosulfide that has been shown to inhibit both the initiation and promotion phases of cancer in vivo and in vitro, as well as reduce the risk of cancer in epidemiological studies. MCF-10A cells, regarded as a normal breast epithelial cell line, were treated with varying concentrations of DES, DAS or various dose combinations of DES and DAS concomitantly, and assessed for cell viability, DNA strand breaks, and lipid peroxidation. DES (10 μM) in combination with 1, 10, or 100 μM DAS resulted in a 31{\%}, 34{\%}, or 36{\%} respective increase in cell viability compared to the DES treatment alone, after 24 h. At the same time point, 1, 10, and 100 μM DAS were all effective in significantly reducing DES (100 μM)-induced strand breaks to near that of the vehicle control. Additionally, 1 μM DAS was effective in significantly reducing DES (100 μM)-induced lipid peroxidation after 3 h. The results of this research suggest that DAS is effective in recovering cell viability, attenuating DNA strand breaks, and decreasing lipid peroxidation in MCF-10A cells.",
keywords = "Breast epithelial cells chemoprevention, DNA damage, Diallyl sulfide, Diethystilbestrol",
author = "McCaskill, {Michael L.} and Rogan, {Eleanor G} and Thomas, {Ronald D.}",
year = "2014",
month = "12",
day = "15",
doi = "10.1016/j.steroids.2014.09.005",
language = "English (US)",
volume = "92",
pages = "96--100",
journal = "Steroids",
issn = "0039-128X",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Diallyl sulfide inhibits diethylstilbestrol induced DNA damage in human breast epithelial cells (MCF-10A)

AU - McCaskill, Michael L.

AU - Rogan, Eleanor G

AU - Thomas, Ronald D.

PY - 2014/12/15

Y1 - 2014/12/15

N2 - Breast cancer is the second leading cause of cancer deaths in women in the United States. Diethylstilbestrol (DES) is a synthetic estrogen that has been shown to cause cancer in animals and humans, altering cell viability as well as inducing DNA damage. Diallyl sulfide (DAS) is a garlic organosulfide that has been shown to inhibit both the initiation and promotion phases of cancer in vivo and in vitro, as well as reduce the risk of cancer in epidemiological studies. MCF-10A cells, regarded as a normal breast epithelial cell line, were treated with varying concentrations of DES, DAS or various dose combinations of DES and DAS concomitantly, and assessed for cell viability, DNA strand breaks, and lipid peroxidation. DES (10 μM) in combination with 1, 10, or 100 μM DAS resulted in a 31%, 34%, or 36% respective increase in cell viability compared to the DES treatment alone, after 24 h. At the same time point, 1, 10, and 100 μM DAS were all effective in significantly reducing DES (100 μM)-induced strand breaks to near that of the vehicle control. Additionally, 1 μM DAS was effective in significantly reducing DES (100 μM)-induced lipid peroxidation after 3 h. The results of this research suggest that DAS is effective in recovering cell viability, attenuating DNA strand breaks, and decreasing lipid peroxidation in MCF-10A cells.

AB - Breast cancer is the second leading cause of cancer deaths in women in the United States. Diethylstilbestrol (DES) is a synthetic estrogen that has been shown to cause cancer in animals and humans, altering cell viability as well as inducing DNA damage. Diallyl sulfide (DAS) is a garlic organosulfide that has been shown to inhibit both the initiation and promotion phases of cancer in vivo and in vitro, as well as reduce the risk of cancer in epidemiological studies. MCF-10A cells, regarded as a normal breast epithelial cell line, were treated with varying concentrations of DES, DAS or various dose combinations of DES and DAS concomitantly, and assessed for cell viability, DNA strand breaks, and lipid peroxidation. DES (10 μM) in combination with 1, 10, or 100 μM DAS resulted in a 31%, 34%, or 36% respective increase in cell viability compared to the DES treatment alone, after 24 h. At the same time point, 1, 10, and 100 μM DAS were all effective in significantly reducing DES (100 μM)-induced strand breaks to near that of the vehicle control. Additionally, 1 μM DAS was effective in significantly reducing DES (100 μM)-induced lipid peroxidation after 3 h. The results of this research suggest that DAS is effective in recovering cell viability, attenuating DNA strand breaks, and decreasing lipid peroxidation in MCF-10A cells.

KW - Breast epithelial cells chemoprevention

KW - DNA damage

KW - Diallyl sulfide

KW - Diethystilbestrol

UR - http://www.scopus.com/inward/record.url?scp=84908692727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908692727&partnerID=8YFLogxK

U2 - 10.1016/j.steroids.2014.09.005

DO - 10.1016/j.steroids.2014.09.005

M3 - Article

C2 - 25278253

AN - SCOPUS:84908692727

VL - 92

SP - 96

EP - 100

JO - Steroids

JF - Steroids

SN - 0039-128X

ER -