Diabetes-induced cerebrovascular dysfunction: Role of poly(ADP-ribose) polymerase

Denise M. Arrick, Glenda M. Sharpe, Hong Sun, William G. Mayhan

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Our goal was to identify the role of poly(ADP-ribose) polymerase (PARP) in cerebrovascular dysfunction in Type 1 diabetes mellitus (T1D). In a first series of studies, rats were assigned to nondiabetic and diabetic (streptozotocin; 50 mg/kg IP) groups. Two to three months after injection of streptozotocin, we examine in vivo responses of pial arterioles to nitric oxide synthase (NOS)-dependent (adenosine diphosphate (ADP), acetylcholine and histamine) and -independent (nitroglycerin) agonists. After the initial examination of reactivity to the agonists, we treated pial arterioles acutely with an inhibitor of PARP (PJ-34; 1 μM), and then we again examined responses to the agonists. In a second series of studies, we examine superoxide production (lucigenin chemiluminescence) by parietal cortex tissue in nondiabetic and diabetic rats. We found that dilation of pial arterioles in response to ADP, acetylcholine and histamine, but not to nitroglycerin, was impaired in diabetic compared to nondiabetic rats. In addition, although PJ-34 did not alter responses in nondiabetic rats, PJ-34 alleviated T1D-induced impairment of NOS-dependent vasodilation. We also found that basal production of superoxide was increased in diabetic compared to nondiabetic rats and that PJ-34 decreased this basal production of superoxide. Our findings suggest that T1D impairs NOS-dependent reactivity of cerebral arterioles by a mechanism that appears to be related to the formation of superoxide via activation of PARP.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalMicrovascular Research
Volume73
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Poly Adenosine Diphosphate Ribose
Medical problems
Rats
Arterioles
Superoxides
Type 1 Diabetes Mellitus
Nitric Oxide Synthase
Nitroglycerin
Streptozocin
Adenosine Diphosphate
Histamine
Acetylcholine
Parietal Lobe
Chemiluminescence
Poly(ADP-ribose) Polymerases
Luminescence
Vasodilation
Dilatation
Chemical activation
Tissue

Keywords

  • Acetylcholine
  • Adenosine diphosphate
  • Arterioles
  • Brain
  • Histamine
  • Microcirculation
  • Nitric oxide
  • Nitroglycerin
  • PARP
  • PJ-34
  • Rats
  • Type I diabetes

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Diabetes-induced cerebrovascular dysfunction : Role of poly(ADP-ribose) polymerase. / Arrick, Denise M.; Sharpe, Glenda M.; Sun, Hong; Mayhan, William G.

In: Microvascular Research, Vol. 73, No. 1, 01.01.2007, p. 1-6.

Research output: Contribution to journalArticle

Arrick, Denise M. ; Sharpe, Glenda M. ; Sun, Hong ; Mayhan, William G. / Diabetes-induced cerebrovascular dysfunction : Role of poly(ADP-ribose) polymerase. In: Microvascular Research. 2007 ; Vol. 73, No. 1. pp. 1-6.
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