Dexamethasone inhibits transforming growth factor-β receptor (TβR) messenger RNA expression in hamster preantral follicles

Possible association with NF-YA

Shyamal K Roy, Jinrong Wang, Peixin Yang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To evaluate the site (s) and mechanism (s) of glucocorticoid-inhibition of transforming growth factor (TGF) β receptor (TβR) mRNA expression in ovarian cells, steady-state levels of TβR mRNA in hamster preantral follicles exposed to FSH or estradiol with or without dexamethasone were determined by reverse transcription polymerase chain reaction and Southern hybridization. The effect of dexamethasone on follicular DNA and steroid synthesis and the expression of NF-Y and Sp3 were also investigated. Dexamethasone differentially inhibited FSH- or estradiol-induced expression of TβR mRNA in preantral follicles at all stages. Dexamethasone also strongly inhibited FSH-induced but not TGFβ2-induced follicular DNA synthesis, and the inhibition was completely reversed by TGFβ2. However, TGFβ2 markedly attenuated FSH + dexamethasone-stimulated progesterone and FSH-induced follicular estradiol synthesis. Both FSH and estradiol upregulated NF-YA expression, but the effect was significantly attenuated by dexamethasone. Our results suggest that suppression of NF-YA levels is one of the mechanisms whereby dexamethasone reduces hormone-induced TβRI and TβRII mRNA levels in hamster preantral follicles. Dexamethasone potentiates the effect of FSH on granulosa cell steroidogenesis, whereas TGFβ counteracts the effect. These data indicate that glucocorticoid and TGFβ may form an important regulatory loop to modulate FSH regulation of preantral follicular growth and differentiation.

Original languageEnglish (US)
Pages (from-to)2180-2188
Number of pages9
JournalBiology of reproduction
Volume68
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Growth Factor Receptors
Transforming Growth Factors
Cricetinae
Dexamethasone
Messenger RNA
Estradiol
Glucocorticoids
Granulosa Cells
DNA
Reverse Transcription
Progesterone
Steroids
Hormones
Polymerase Chain Reaction
Growth

Keywords

  • Estradiol
  • Follicle
  • Follicular development
  • Growth factors
  • Ovary

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Dexamethasone inhibits transforming growth factor-β receptor (TβR) messenger RNA expression in hamster preantral follicles : Possible association with NF-YA. / Roy, Shyamal K; Wang, Jinrong; Yang, Peixin.

In: Biology of reproduction, Vol. 68, No. 6, 01.06.2003, p. 2180-2188.

Research output: Contribution to journalArticle

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