Developmental regulation of spine motility in the mammalian central nervous system

Anna Dunaevsky, Ayumu Tashiro, Ania Majewska, Carol Mason, Rafael Yuste

Research output: Contribution to journalArticle

330 Citations (Scopus)

Abstract

The function of dendritic spines, postsynaptic sites of excitatory input in the mammalian central nervous system (CNS), is still not well understood. Although changes in spine morphology may mediate synaptic plasticity, the extent of basal spine motility and its regulation and function remains controversial. We investigated spine motility in three principal neurons of the mouse CNS: cerebellar Purkinje cells, and cortical and hippocampal pyramidal neurons. Motility was assayed with time-lapse imaging by using two- photon microscopy of green fluorescent protein-labeled neurons in acute and cultured slices. In all three cell types, dendritic protrusions (filopodia and spines) were highly dynamic, exhibiting a diversity of morphological rearrangements over short (<1-min) time courses. The incidence of spine motility declined during postnatal maturation, but dynamic changes were still apparent in many spines in late-postnatal neurons. Although blockade or induction of neuronal activity did not affect spine motility, disruption of actin polymerization did. We hypothesize that this basal motility of dendritic protrusions is intrinsic to the neuron and underlies the heightened plasticity found in developing CNS.

Original languageEnglish (US)
Pages (from-to)13438-13443
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number23
DOIs
StatePublished - Nov 9 1999

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Spine
Central Nervous System
Neurons
Time-Lapse Imaging
Dendritic Spines
Pseudopodia
Neuronal Plasticity
Pyramidal Cells
Purkinje Cells
Green Fluorescent Proteins
Photons
Polymerization
Dendritic Cells
Actins
Microscopy
Incidence

ASJC Scopus subject areas

  • General

Cite this

Developmental regulation of spine motility in the mammalian central nervous system. / Dunaevsky, Anna; Tashiro, Ayumu; Majewska, Ania; Mason, Carol; Yuste, Rafael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 23, 09.11.1999, p. 13438-13443.

Research output: Contribution to journalArticle

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