Developmental regulation of hemoglobin synthesis in the green anole lizard Anolis carolinensis

Jay F Storz, Federico G. Hoffmann, Juan C. Opazo, Thomas J. Sanger, Hideaki Moriyama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tetrapod vertebrates possess multiple α-and β-like globin genes that are ontogenetically regulated, such that functionally distinct hemoglobin (Hb) isoforms are synthesized during different stages of development. The α-and β-like globin genes of amphibians, birds and mammals are differentially expressed during embryonic development and postnatal life, but little is known about the developmental regulation of globin gene expression in non-avian reptiles. Here we report an investigation into the developmental regulation of Hb synthesis in the green anole lizard Anolis carolinensis. We tested two hypotheses derived from comparative genomic studies of the globin gene clusters in tetrapod vertebrates. First, we tested whether the product of the Anolis αD-globin gene is incorporated into embryonic Hb, thereby performing the role that would normally be performed by the embyronic αE-globin gene (which has been deleted from the green anole genome). Second, we tested whether two 'lizard-specific' β-globin paralogs have independently evolved a division of labor between an early-expressed embryonic gene and a later-expressed adult gene. Results of a proteomic analysis revealed that α-and β-like globin genes of the anole are differentially expressed during embryonic development. However, the same repertoire of α-and β-chain Hb isoforms was expressed during all stages of development and postnatal life, and the ontogenetic shifts in isoform composition were relatively subtle. In contrast to the pattern that has been documented in other tetrapod vertebrates, it appears that the developmental regulation of Hb synthesis in the green anole lizard does not involve discrete, stage-specific switches in gene activation and gene silencing.

Original languageEnglish (US)
Pages (from-to)575-581
Number of pages7
JournalJournal of Experimental Biology
Volume214
Issue number4
DOIs
StatePublished - Feb 1 2011

Fingerprint

Anolis carolinensis
Lizards
Globins
hemoglobin
lizard
Hemoglobins
synthesis
gene
Genes
genes
tetrapod
Vertebrates
vertebrates
Protein Isoforms
vertebrate
embryogenesis
Embryonic Development
Developmental Gene Expression Regulation
embryonic development
Switch Genes

Keywords

  • Anolis
  • Gene duplication
  • Globin gene family
  • Hemoglobin
  • Reptile genomics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

Cite this

Developmental regulation of hemoglobin synthesis in the green anole lizard Anolis carolinensis. / Storz, Jay F; Hoffmann, Federico G.; Opazo, Juan C.; Sanger, Thomas J.; Moriyama, Hideaki.

In: Journal of Experimental Biology, Vol. 214, No. 4, 01.02.2011, p. 575-581.

Research output: Contribution to journalArticle

Storz, Jay F ; Hoffmann, Federico G. ; Opazo, Juan C. ; Sanger, Thomas J. ; Moriyama, Hideaki. / Developmental regulation of hemoglobin synthesis in the green anole lizard Anolis carolinensis. In: Journal of Experimental Biology. 2011 ; Vol. 214, No. 4. pp. 575-581.
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