Developmental changes in glycoconjugate secretion by ferret tracheas

M. W. Leigh, P. W. Cheng, J. L. Carson, T. F. Boat

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Surface epithelium and submucosal glands of the ferret trachea undergo extensive postnatal development. This study examined developmental changes in rates of release and types of high molecular weight glycoconjugates secreted by explanted ferret tracheas. Digestion with bovine testicular hyaluronidase separated the high molecular weight glycoconjugates into two types, hyaluronidase-resistant mucins and hyaluronidase-susceptible glycosaminoglycans. Release rates were measured under unstimulated conditions and in the presence of known secretagogues. The unstimulated rate of release of total 3H-glycoconjugates was 4-fold higher at birth than after complete maturation. The mucin content varied from 39 to 74% of total 3H-glycoconjugates; however, no age-related pattern was observed for mucin/glycosaminoglycan ratios. The rate of release of 3H-mucins was 6-fold higher at birth than in the adult but rapidly dropped to adult levels by 28 days of age. The secretory cells in the tracheal epithelium of newborn ferrets had more abundant rough endoplasmic reticulum than did mature goblet cells, suggesting increased synthesis of secretory product. Response to methacholine and trypsin, both known stimulators of mucin release, was not observed until 28 and 54 days of age, respectively. Incorporation of 35S-sulfate into mucins relative to that for 3H-glucosamine increased with age, consistent with increasingly acidic histochemical staining properties of secretory cells. These developmental differences in rates of release, modulation of release, and relative sulfation of mucins may represent changes in secretory and synthetic mechanisms of the secretory cells.

Original languageEnglish (US)
Pages (from-to)784-790
Number of pages7
JournalAmerican Review of Respiratory Disease
Volume134
Issue number4
StatePublished - Jan 1 1986

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Ferrets
Glycoconjugates
Mucins
Trachea
Hyaluronoglucosaminidase
Glycosaminoglycans
Mucin-6
Epithelium
Molecular Weight
Parturition
Goblet Cells
Methacholine Chloride
Rough Endoplasmic Reticulum
Glucosamine
Trypsin
Sulfates
Digestion
Staining and Labeling

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Developmental changes in glycoconjugate secretion by ferret tracheas. / Leigh, M. W.; Cheng, P. W.; Carson, J. L.; Boat, T. F.

In: American Review of Respiratory Disease, Vol. 134, No. 4, 01.01.1986, p. 784-790.

Research output: Contribution to journalArticle

Leigh, M. W. ; Cheng, P. W. ; Carson, J. L. ; Boat, T. F. / Developmental changes in glycoconjugate secretion by ferret tracheas. In: American Review of Respiratory Disease. 1986 ; Vol. 134, No. 4. pp. 784-790.
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