Development of murine embryos following electroporation

C. A. Schmotzer, M. E. Dunlap-Brown, S. P. Butler, W. H. Velander, F. C. Gwazdauskas

Research output: Contribution to journalArticle

Abstract

Purpose: The objective was to establish the parameters for reversible electroporation of murine embryos. Methods: In Trial 1, murine presumptive zygotes received an electrical pulse of 5, 10, or 20-μs duration, and one of five voltages (100, 200, 250, 300, or 400 V). In Trial 2, embryo orientation within the electroporation chamber was evaluated with 250 or 400 V at a pulse period of 10 μs. Results: Presumptive zygotes that received 400 V at each pulse length and zygotes exposed to 20 μs at each voltage had the lowest embryonic development (P < 0.05). Presumptive zygotes that received 250 V had higher development compared to 400 V, irrespective of orientation (P < 0.01), but development was lower than the controls (P < 0.01). Conclusions: Electrical stimulation of presumptive zygotes can have a detrimental impact on early embryo development, but low amounts of stimulation may allow for potential gene transfer in transgenic experimentation.

Original languageEnglish (US)
Pages (from-to)148-152
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Volume20
Issue number4
DOIs
StatePublished - Apr 1 2003

Fingerprint

Electroporation
Zygote
Embryonic Development
Embryonic Structures
Electric Stimulation
Genes

Keywords

  • Electroporation
  • Murine
  • Presumptive zygotes
  • Pulse period
  • Voltage

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

Development of murine embryos following electroporation. / Schmotzer, C. A.; Dunlap-Brown, M. E.; Butler, S. P.; Velander, W. H.; Gwazdauskas, F. C.

In: Journal of Assisted Reproduction and Genetics, Vol. 20, No. 4, 01.04.2003, p. 148-152.

Research output: Contribution to journalArticle

Schmotzer, CA, Dunlap-Brown, ME, Butler, SP, Velander, WH & Gwazdauskas, FC 2003, 'Development of murine embryos following electroporation', Journal of Assisted Reproduction and Genetics, vol. 20, no. 4, pp. 148-152. https://doi.org/10.1023/A:1022929119792
Schmotzer, C. A. ; Dunlap-Brown, M. E. ; Butler, S. P. ; Velander, W. H. ; Gwazdauskas, F. C. / Development of murine embryos following electroporation. In: Journal of Assisted Reproduction and Genetics. 2003 ; Vol. 20, No. 4. pp. 148-152.
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AU - Butler, S. P.

AU - Velander, W. H.

AU - Gwazdauskas, F. C.

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