Development of glucogenesis from galactose by fetal rat liver explants in organ culture

Ronald A. Simkins, Howard J. Eisen, John W Sparks, Walter H. Glinsmann

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Basic parameters of net glucose and net galactose uptake by fetal rat liver explants at 16 and 20 days of gestation (term = 21-22 days) remained relatively constant over 3 days in a defined organ culture system. Explants at 20 days of gestation released glucose into the culture medium in proportion to the varied medium galactose concentrations (0 to 20 mM); explants at 16 days of gestation lacked this glucogenic response. When 16-day explants were exposed to glucocorticoids (triamcinolone, dexamethasone, or corticosterone) during culture, there was a time- and concentration-dependent development of glucose production from galactose. The precocious development of galactose glucogenesis could be reproduced in utero by the interperitoneal injection of 0.4 mg of dexamethasone into mothers on Days 14 and 15 of gestation. Such 16-day explants from fetuses of dexamethasone-treated mothers exhibited a further increase in the magnitude of glucose released when they were exposed to additional glucocorticoids in culture. Other steroid hormones (progesterone, estradiol, and testosterone) as well as glucagon had no effect on the development of this response.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalDevelopmental Biology
Volume66
Issue number2
DOIs
StatePublished - Jan 1 1978

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Organ Culture Techniques
Galactose
Dexamethasone
Glucose
Pregnancy
Liver
Glucocorticoids
Triamcinolone
Corticosterone
Glucagon
Progesterone
Culture Media
Testosterone
Estradiol
Fetus
Steroids
Hormones
Injections

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Development of glucogenesis from galactose by fetal rat liver explants in organ culture. / Simkins, Ronald A.; Eisen, Howard J.; Sparks, John W; Glinsmann, Walter H.

In: Developmental Biology, Vol. 66, No. 2, 01.01.1978, p. 353-360.

Research output: Contribution to journalArticle

Simkins, Ronald A. ; Eisen, Howard J. ; Sparks, John W ; Glinsmann, Walter H. / Development of glucogenesis from galactose by fetal rat liver explants in organ culture. In: Developmental Biology. 1978 ; Vol. 66, No. 2. pp. 353-360.
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