Development of Cryogel-Based Guidance Conduit for Peripheral Nerve Regeneration

Shaohua Wu, Mitchell Kuss, Dianjun Qi, Juan Hong, Han Jun Wang, Wenhai Zhang, Shaojuan Chen, Shilei Ni, Bin Duan

Research output: Contribution to journalArticle

Abstract

Development of a nerve guidance conduit (NGC) is critically required for replacing the gold standard, autograft, in the repair of peripheral nerve (PN) injury with a long transecting gap. In the present study, an engineered cryogel-based NGC composed of methacrylated gelatin (MeGel), methacrylated hyaluronic acid (MeHA), and 4 arm poly(ethylene glycol) acrylate (4Arm-PEG-acrylate) was fabricated by utilizing a free radical cryopolymerization method. In vitro studies showed that the MeGel/MeHA/4Arm-PEG-acrylate hybrid cryogel provided a favorable surface and microenvironment for the adhesion, proliferation, and phenotype maintenance of rabbit Schwann cells. A 10 mm long transection in the rat sciatic nerve was created for in vivo studies. The results demonstrated that after the 16 weeks of repairing, the cryogel-based NGC supported axon regeneration and remyelination with comparable myelinating diameter and density of the regenerated nerve fibers to the autograft group. The rats with implanted cryogel-based NGC also showed sensory function recovery. This study demonstrated that our cryogel-based NGC is effective at assisting the repair of damaged PN because of its excellent structural stability, great handling convenience, and regeneration guidance capacity.

Original languageEnglish (US)
Pages (from-to)4864-4871
Number of pages8
JournalACS Applied Bio Materials
Volume2
Issue number11
DOIs
StatePublished - Nov 18 2019

Fingerprint

Cryogels
Hyaluronic acid
Nerve Regeneration
Peripheral Nerves
Polyethylene glycols
Rats
Repair
Free radicals
Ethylene Glycol
Autografts
Hyaluronic Acid
Gelatin
Adhesion
Cells
Regeneration
Recovery
Arm
Fibers
Peripheral Nerve Injuries
Schwann Cells

Keywords

  • Schwann cells
  • cryopolymerization
  • hydrogel
  • nerve repair
  • neural tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

Cite this

Development of Cryogel-Based Guidance Conduit for Peripheral Nerve Regeneration. / Wu, Shaohua; Kuss, Mitchell; Qi, Dianjun; Hong, Juan; Wang, Han Jun; Zhang, Wenhai; Chen, Shaojuan; Ni, Shilei; Duan, Bin.

In: ACS Applied Bio Materials, Vol. 2, No. 11, 18.11.2019, p. 4864-4871.

Research output: Contribution to journalArticle

Wu, S, Kuss, M, Qi, D, Hong, J, Wang, HJ, Zhang, W, Chen, S, Ni, S & Duan, B 2019, 'Development of Cryogel-Based Guidance Conduit for Peripheral Nerve Regeneration', ACS Applied Bio Materials, vol. 2, no. 11, pp. 4864-4871. https://doi.org/10.1021/acsabm.9b00626
Wu, Shaohua ; Kuss, Mitchell ; Qi, Dianjun ; Hong, Juan ; Wang, Han Jun ; Zhang, Wenhai ; Chen, Shaojuan ; Ni, Shilei ; Duan, Bin. / Development of Cryogel-Based Guidance Conduit for Peripheral Nerve Regeneration. In: ACS Applied Bio Materials. 2019 ; Vol. 2, No. 11. pp. 4864-4871.
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