Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells

P. Panigrahi, B. D. Tall, R. G. Russell, L. J. Detolla, J. G. Morris

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Non-O1 Vibrio cholerae strains have been reported as a causative agent of diarrhea throughout the world. We recently reported that non-O1 V. cholerae strains cause diarrhea in human volunteers. In this study we evaluated the virulence of three strains of non-O1 V. cholerae in a Caco-2 cell adherence assay by light and electron microscopy. A-5 is an environmental isolate which failed to colonize volunteers and did not cause diarrhea. It exhibited low numbers of organisms adherent to Caco-2 cells, leaving the microvilli intact. Strain 2076-79, isolated from a patient with diarrhea, colonized human volunteers without producing disease. It adhered to Caco-2 cells in moderate numbers without producing any damage to the microvilli. Strain NRT36S, a clinical isolate, colonized human volunteers and produced significantly diarrheal disease. This strain adhered in very large numbers to Caco-2 cells and caused damage to the brush borders. Membrane-bound bacteria were also seen within the cytoplasm of these cells. Scanning electron microscopy confirmed the generalized adherence of NRT36S to the microvilli of Caco-2 cells. The three strains did not appear to compete with each other for binding sites on Caco-2 cells and were not adherent when assays were conducted at 4°C. Our results with strains A-5, 2076-79, and NRT36S correlate well with observations in human volunteer studies, suggesting that Caco-2 cells provide an appropriate in vitro system for further investigation of the pathogenesis of non-O1 V. cholerae gastroenteritis.

Original languageEnglish (US)
Pages (from-to)3415-3424
Number of pages10
JournalInfection and immunity
Volume58
Issue number10
StatePublished - Jan 1 1990

Fingerprint

Vibrio cholerae non-O1
Caco-2 Cells
Virulence
Volunteers
Microvilli
Diarrhea
Gastroenteritis
In Vitro Techniques
Electron Scanning Microscopy
Electron Microscopy
Cytoplasm
Binding Sites
Bacteria
Light
Membranes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Panigrahi, P., Tall, B. D., Russell, R. G., Detolla, L. J., & Morris, J. G. (1990). Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells. Infection and immunity, 58(10), 3415-3424.

Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells. / Panigrahi, P.; Tall, B. D.; Russell, R. G.; Detolla, L. J.; Morris, J. G.

In: Infection and immunity, Vol. 58, No. 10, 01.01.1990, p. 3415-3424.

Research output: Contribution to journalArticle

Panigrahi, P, Tall, BD, Russell, RG, Detolla, LJ & Morris, JG 1990, 'Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells', Infection and immunity, vol. 58, no. 10, pp. 3415-3424.
Panigrahi P, Tall BD, Russell RG, Detolla LJ, Morris JG. Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells. Infection and immunity. 1990 Jan 1;58(10):3415-3424.
Panigrahi, P. ; Tall, B. D. ; Russell, R. G. ; Detolla, L. J. ; Morris, J. G. / Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells. In: Infection and immunity. 1990 ; Vol. 58, No. 10. pp. 3415-3424.
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