Development of a reliable model of total abdominal wall transplantation

Meghan A. Quigley, Derek R. Fletcher, Wensheng Zhang, Vu T. Nguyen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: Among the most common complications associated with abdominal organ transplantation are issues with abdominal wall closure. This difficulty, along with the recent rise in the use of vascularized composite allotransplantation, has led surgeons to the notion of abdominal wall transplantation. METHODS: The authors have developed a novel surgical model for a full-thickness total abdominal wall transplant based on a unilateral pedicle between fully major histocompatibility complex-mismatched rat strains. An established cuff technique was used to anastomose the donor's common iliac vessels to the recipient's femoral vessels. Three groups (n = 6 per group) received FK506 monotherapy, 0.25, 0.50, or 0.75 mg/kg per day. The transplants were assessed daily for evidence of rejection by visual inspection. Hematoxylin and eosin skin biopsies and fluorescence-activated cell sorting analysis of the recipients' peripheral blood were performed. RESULTS: No signs of rejection were observed in the animals receiving FK506, 0.50 or 0.75 mg/kg per day. Those receiving 0.25 mg/kg per day, however, displayed a mixed rejection response. Flow cytometric analysis did not show evidence of donor-specific chimerism. CONCLUSIONS: A full-thickness abdominal wall graft based on a unilateral pedicle is a technically feasible and reliable small-animal surgical model. The use of greater than or equal to 0.50 mg/kg per day FK506 results in 100 percent graft survival. Future plans are to use this model to investigate the immunologic interaction of a concomitant solid organ and an abdominal wall allograft, as would likely occur in the clinical situation.

Original languageEnglish (US)
Pages (from-to)988-994
Number of pages7
JournalPlastic and Reconstructive Surgery
Volume132
Issue number4
DOIs
StatePublished - Oct 1 2013

Fingerprint

Abdominal Wall
Transplantation
Tacrolimus
Anatomic Models
Transplants
Vascularized Composite Allotransplantation
Tissue Donors
Chimerism
Organ Transplantation
Graft Survival
Hematoxylin
Eosine Yellowish-(YS)
Thigh
Major Histocompatibility Complex
Allografts
Flow Cytometry
Animal Models
Biopsy
Skin

ASJC Scopus subject areas

  • Surgery

Cite this

Development of a reliable model of total abdominal wall transplantation. / Quigley, Meghan A.; Fletcher, Derek R.; Zhang, Wensheng; Nguyen, Vu T.

In: Plastic and Reconstructive Surgery, Vol. 132, No. 4, 01.10.2013, p. 988-994.

Research output: Contribution to journalArticle

Quigley, Meghan A. ; Fletcher, Derek R. ; Zhang, Wensheng ; Nguyen, Vu T. / Development of a reliable model of total abdominal wall transplantation. In: Plastic and Reconstructive Surgery. 2013 ; Vol. 132, No. 4. pp. 988-994.
@article{91c833a7096d48c2a89c79cd12cfb1ba,
title = "Development of a reliable model of total abdominal wall transplantation",
abstract = "BACKGROUND: Among the most common complications associated with abdominal organ transplantation are issues with abdominal wall closure. This difficulty, along with the recent rise in the use of vascularized composite allotransplantation, has led surgeons to the notion of abdominal wall transplantation. METHODS: The authors have developed a novel surgical model for a full-thickness total abdominal wall transplant based on a unilateral pedicle between fully major histocompatibility complex-mismatched rat strains. An established cuff technique was used to anastomose the donor's common iliac vessels to the recipient's femoral vessels. Three groups (n = 6 per group) received FK506 monotherapy, 0.25, 0.50, or 0.75 mg/kg per day. The transplants were assessed daily for evidence of rejection by visual inspection. Hematoxylin and eosin skin biopsies and fluorescence-activated cell sorting analysis of the recipients' peripheral blood were performed. RESULTS: No signs of rejection were observed in the animals receiving FK506, 0.50 or 0.75 mg/kg per day. Those receiving 0.25 mg/kg per day, however, displayed a mixed rejection response. Flow cytometric analysis did not show evidence of donor-specific chimerism. CONCLUSIONS: A full-thickness abdominal wall graft based on a unilateral pedicle is a technically feasible and reliable small-animal surgical model. The use of greater than or equal to 0.50 mg/kg per day FK506 results in 100 percent graft survival. Future plans are to use this model to investigate the immunologic interaction of a concomitant solid organ and an abdominal wall allograft, as would likely occur in the clinical situation.",
author = "Quigley, {Meghan A.} and Fletcher, {Derek R.} and Wensheng Zhang and Nguyen, {Vu T.}",
year = "2013",
month = "10",
day = "1",
doi = "10.1097/PRS.0b013e31829f4bd3",
language = "English (US)",
volume = "132",
pages = "988--994",
journal = "Plastic and Reconstructive Surgery",
issn = "0032-1052",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Development of a reliable model of total abdominal wall transplantation

AU - Quigley, Meghan A.

AU - Fletcher, Derek R.

AU - Zhang, Wensheng

AU - Nguyen, Vu T.

PY - 2013/10/1

Y1 - 2013/10/1

N2 - BACKGROUND: Among the most common complications associated with abdominal organ transplantation are issues with abdominal wall closure. This difficulty, along with the recent rise in the use of vascularized composite allotransplantation, has led surgeons to the notion of abdominal wall transplantation. METHODS: The authors have developed a novel surgical model for a full-thickness total abdominal wall transplant based on a unilateral pedicle between fully major histocompatibility complex-mismatched rat strains. An established cuff technique was used to anastomose the donor's common iliac vessels to the recipient's femoral vessels. Three groups (n = 6 per group) received FK506 monotherapy, 0.25, 0.50, or 0.75 mg/kg per day. The transplants were assessed daily for evidence of rejection by visual inspection. Hematoxylin and eosin skin biopsies and fluorescence-activated cell sorting analysis of the recipients' peripheral blood were performed. RESULTS: No signs of rejection were observed in the animals receiving FK506, 0.50 or 0.75 mg/kg per day. Those receiving 0.25 mg/kg per day, however, displayed a mixed rejection response. Flow cytometric analysis did not show evidence of donor-specific chimerism. CONCLUSIONS: A full-thickness abdominal wall graft based on a unilateral pedicle is a technically feasible and reliable small-animal surgical model. The use of greater than or equal to 0.50 mg/kg per day FK506 results in 100 percent graft survival. Future plans are to use this model to investigate the immunologic interaction of a concomitant solid organ and an abdominal wall allograft, as would likely occur in the clinical situation.

AB - BACKGROUND: Among the most common complications associated with abdominal organ transplantation are issues with abdominal wall closure. This difficulty, along with the recent rise in the use of vascularized composite allotransplantation, has led surgeons to the notion of abdominal wall transplantation. METHODS: The authors have developed a novel surgical model for a full-thickness total abdominal wall transplant based on a unilateral pedicle between fully major histocompatibility complex-mismatched rat strains. An established cuff technique was used to anastomose the donor's common iliac vessels to the recipient's femoral vessels. Three groups (n = 6 per group) received FK506 monotherapy, 0.25, 0.50, or 0.75 mg/kg per day. The transplants were assessed daily for evidence of rejection by visual inspection. Hematoxylin and eosin skin biopsies and fluorescence-activated cell sorting analysis of the recipients' peripheral blood were performed. RESULTS: No signs of rejection were observed in the animals receiving FK506, 0.50 or 0.75 mg/kg per day. Those receiving 0.25 mg/kg per day, however, displayed a mixed rejection response. Flow cytometric analysis did not show evidence of donor-specific chimerism. CONCLUSIONS: A full-thickness abdominal wall graft based on a unilateral pedicle is a technically feasible and reliable small-animal surgical model. The use of greater than or equal to 0.50 mg/kg per day FK506 results in 100 percent graft survival. Future plans are to use this model to investigate the immunologic interaction of a concomitant solid organ and an abdominal wall allograft, as would likely occur in the clinical situation.

UR - http://www.scopus.com/inward/record.url?scp=84885441336&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885441336&partnerID=8YFLogxK

U2 - 10.1097/PRS.0b013e31829f4bd3

DO - 10.1097/PRS.0b013e31829f4bd3

M3 - Article

C2 - 24076687

AN - SCOPUS:84885441336

VL - 132

SP - 988

EP - 994

JO - Plastic and Reconstructive Surgery

JF - Plastic and Reconstructive Surgery

SN - 0032-1052

IS - 4

ER -