Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue

Steven D. Sorden, Perry A. Harms, Porntippa Nawagitgul, David Cavanaugh, Prem S. Paul

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

An immunohistochemical method for the detection of type 2 porcine circovirus (PCV2) in paraffin-embedded tissue was developed. Rabbits were inoculated with purified PCV2 to obtain a polyclonal antiserum. Antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which PCV2 genome had been demonstrated by in situ hybridization. In all cases (18/18), the density and distribution of positive cells detected by in situ hybridization or immunohistochemistry were identical. The immunohistochemical method is more rapid and less expensive than in situ hybridization and is thus more suitable for routine diagnostic use.

Original languageEnglish (US)
Pages (from-to)528-530
Number of pages3
JournalJournal of Veterinary Diagnostic Investigation
Volume11
Issue number6
DOIs
StatePublished - Nov 1999

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Circovirus
Porcine circovirus-2
polyclonal antibodies
formalin
Paraffin
alkanes
in situ hybridization
Formaldehyde
In Situ Hybridization
antiserum
Antibodies
Immune Sera
Wasting Syndrome
postweaning multisystemic wasting syndrome
lesions (animal)
immunohistochemistry
Swine
Immunohistochemistry
methodology
rabbits

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. / Sorden, Steven D.; Harms, Perry A.; Nawagitgul, Porntippa; Cavanaugh, David; Paul, Prem S.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 6, 11.1999, p. 528-530.

Research output: Contribution to journalArticle

Sorden, Steven D. ; Harms, Perry A. ; Nawagitgul, Porntippa ; Cavanaugh, David ; Paul, Prem S. / Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. In: Journal of Veterinary Diagnostic Investigation. 1999 ; Vol. 11, No. 6. pp. 528-530.
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