Development of a novel method of lytic phage delivery by use of a bacteriophage P22 site-specific recombination system

Ratree Platt, Donald Reynolds, Gregory J. Phillips

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bacteriophage therapy represents a potential alternative to the use of antibiotics to control proliferation of pathogenic bacteria. As an alternative to the strategy where a limited number of doses of large numbers of lytic bacteriophages are administered, a novel method delivery system was developed so that phages are continually released into the culture. Specifically, a non-pathogenic Escherichia coli strain was constructed that was lysogenic for a lytic mutant of bacteriophage λ. This lysogen was shown to be effective at decreasing the number of λ-sensitive E. coli in vitro. Construction of this E. coli strain was accomplished by development of a plasmid-based system utilizing the site-specific recombination machinery of bacteriophage P22 to integrate DNA constructs into the host chromosome. This recombination system is useful for strain construction and other genetic manipulations in both E. coli and Salmonella enterica serovars.

Original languageEnglish (US)
Pages (from-to)259-265
Number of pages7
JournalFEMS Microbiology Letters
Volume223
Issue number2
DOIs
StatePublished - Jun 27 2003

Fingerprint

Bacteriophage P22
Bacteriophages
Genetic Recombination
Escherichia coli
Salmonella enterica
Plasmids
Chromosomes
Anti-Bacterial Agents
Bacteria
DNA

Keywords

  • Bacteriophage therapy
  • Excision
  • Integration
  • Lambda

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Development of a novel method of lytic phage delivery by use of a bacteriophage P22 site-specific recombination system. / Platt, Ratree; Reynolds, Donald; Phillips, Gregory J.

In: FEMS Microbiology Letters, Vol. 223, No. 2, 27.06.2003, p. 259-265.

Research output: Contribution to journalArticle

@article{596f7737f30f460fb4706478335f7db9,
title = "Development of a novel method of lytic phage delivery by use of a bacteriophage P22 site-specific recombination system",
abstract = "Bacteriophage therapy represents a potential alternative to the use of antibiotics to control proliferation of pathogenic bacteria. As an alternative to the strategy where a limited number of doses of large numbers of lytic bacteriophages are administered, a novel method delivery system was developed so that phages are continually released into the culture. Specifically, a non-pathogenic Escherichia coli strain was constructed that was lysogenic for a lytic mutant of bacteriophage λ. This lysogen was shown to be effective at decreasing the number of λ-sensitive E. coli in vitro. Construction of this E. coli strain was accomplished by development of a plasmid-based system utilizing the site-specific recombination machinery of bacteriophage P22 to integrate DNA constructs into the host chromosome. This recombination system is useful for strain construction and other genetic manipulations in both E. coli and Salmonella enterica serovars.",
keywords = "Bacteriophage therapy, Excision, Integration, Lambda",
author = "Ratree Platt and Donald Reynolds and Phillips, {Gregory J.}",
year = "2003",
month = "6",
day = "27",
doi = "10.1016/S0378-1097(03)00388-4",
language = "English (US)",
volume = "223",
pages = "259--265",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Development of a novel method of lytic phage delivery by use of a bacteriophage P22 site-specific recombination system

AU - Platt, Ratree

AU - Reynolds, Donald

AU - Phillips, Gregory J.

PY - 2003/6/27

Y1 - 2003/6/27

N2 - Bacteriophage therapy represents a potential alternative to the use of antibiotics to control proliferation of pathogenic bacteria. As an alternative to the strategy where a limited number of doses of large numbers of lytic bacteriophages are administered, a novel method delivery system was developed so that phages are continually released into the culture. Specifically, a non-pathogenic Escherichia coli strain was constructed that was lysogenic for a lytic mutant of bacteriophage λ. This lysogen was shown to be effective at decreasing the number of λ-sensitive E. coli in vitro. Construction of this E. coli strain was accomplished by development of a plasmid-based system utilizing the site-specific recombination machinery of bacteriophage P22 to integrate DNA constructs into the host chromosome. This recombination system is useful for strain construction and other genetic manipulations in both E. coli and Salmonella enterica serovars.

AB - Bacteriophage therapy represents a potential alternative to the use of antibiotics to control proliferation of pathogenic bacteria. As an alternative to the strategy where a limited number of doses of large numbers of lytic bacteriophages are administered, a novel method delivery system was developed so that phages are continually released into the culture. Specifically, a non-pathogenic Escherichia coli strain was constructed that was lysogenic for a lytic mutant of bacteriophage λ. This lysogen was shown to be effective at decreasing the number of λ-sensitive E. coli in vitro. Construction of this E. coli strain was accomplished by development of a plasmid-based system utilizing the site-specific recombination machinery of bacteriophage P22 to integrate DNA constructs into the host chromosome. This recombination system is useful for strain construction and other genetic manipulations in both E. coli and Salmonella enterica serovars.

KW - Bacteriophage therapy

KW - Excision

KW - Integration

KW - Lambda

UR - http://www.scopus.com/inward/record.url?scp=0038434048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038434048&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(03)00388-4

DO - 10.1016/S0378-1097(03)00388-4

M3 - Article

VL - 223

SP - 259

EP - 265

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -