Development of a novel experimental in vitro model of isothiocyanate-induced apoptosis in human malignant melanoma cells

Theodora Mantso, Aristeidis P. Sfakianos, Aithne Atkinson, Ioannis Anestopoulos, Melina Mitsiogianni, Sotirios Botaitis, Sebachedin Perente, Constantinos Simopoulos, Stavros Vasileiadis, Rodrigo Franco-Cruz, Aglaia Pappa, Mihalis I. Panayiotidis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Isothiocyanates are constituents of cruciferous vegetables which have been associated with reduced cancer risk partially through their ability to induce apoptosis in malignant cells including melanoma. Materials and Methods: We have utilized human malignant melanoma (A375), epidermoid carcinoma (A431) and immortalized keratinocyte (HaCaT) cells exposed to various isothiocyanates, under different experimental conditions. Results: An experimental in vitro model utilizing low isothiocyanate concentrations (0.1-5 μM for 48 h with all treatments being refreshed after 24h) was shown to be (i) most efficient in exerting an anti-cancer effect when compared to higher concentrations (5-100 μM for 24 or 48 h added as a single bolus) and (ii) specific to A375 cells while A431 and HaCaT cells remained unaffected. Such effect involved the activation of several caspases including (iii) initiator caspases 8, 9, 4 (indicating the involvement of intrinsic, extrinsic and endoplasmic reticulum-based pathways) and (iv) effector caspases 3, 7 and 6. Conclusion: Utilization of low isothiocyanate concentrations (under the conditions described herein) exerts an anti-cancer effect specific to human malignant melanoma cells thus providing a therapeutic basis for their utilization in management of the disease.

Original languageEnglish (US)
Pages (from-to)6303-6309
Number of pages7
JournalAnticancer Research
Volume36
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Melanoma
Apoptosis
Isothiocyanates
Initiator Caspases
Caspase 6
Effector Caspases
Caspase 7
Neoplasms
Caspase 9
Caspase 8
Disease Management
Caspases
Keratinocytes
Caspase 3
Endoplasmic Reticulum
Vegetables
Squamous Cell Carcinoma
In Vitro Techniques
isothiocyanic acid
Therapeutics

Keywords

  • Apoptosis
  • Benzyl isothiocyanate
  • Isothiocyanates
  • Malignant melanoma
  • Phenethyl isothiocyanate
  • Sulforaphane

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Mantso, T., Sfakianos, A. P., Atkinson, A., Anestopoulos, I., Mitsiogianni, M., Botaitis, S., ... Panayiotidis, M. I. (2016). Development of a novel experimental in vitro model of isothiocyanate-induced apoptosis in human malignant melanoma cells. Anticancer Research, 36(12), 6303-6309. https://doi.org/10.21873/anticanres.11226

Development of a novel experimental in vitro model of isothiocyanate-induced apoptosis in human malignant melanoma cells. / Mantso, Theodora; Sfakianos, Aristeidis P.; Atkinson, Aithne; Anestopoulos, Ioannis; Mitsiogianni, Melina; Botaitis, Sotirios; Perente, Sebachedin; Simopoulos, Constantinos; Vasileiadis, Stavros; Franco-Cruz, Rodrigo; Pappa, Aglaia; Panayiotidis, Mihalis I.

In: Anticancer Research, Vol. 36, No. 12, 01.12.2016, p. 6303-6309.

Research output: Contribution to journalArticle

Mantso, T, Sfakianos, AP, Atkinson, A, Anestopoulos, I, Mitsiogianni, M, Botaitis, S, Perente, S, Simopoulos, C, Vasileiadis, S, Franco-Cruz, R, Pappa, A & Panayiotidis, MI 2016, 'Development of a novel experimental in vitro model of isothiocyanate-induced apoptosis in human malignant melanoma cells', Anticancer Research, vol. 36, no. 12, pp. 6303-6309. https://doi.org/10.21873/anticanres.11226
Mantso, Theodora ; Sfakianos, Aristeidis P. ; Atkinson, Aithne ; Anestopoulos, Ioannis ; Mitsiogianni, Melina ; Botaitis, Sotirios ; Perente, Sebachedin ; Simopoulos, Constantinos ; Vasileiadis, Stavros ; Franco-Cruz, Rodrigo ; Pappa, Aglaia ; Panayiotidis, Mihalis I. / Development of a novel experimental in vitro model of isothiocyanate-induced apoptosis in human malignant melanoma cells. In: Anticancer Research. 2016 ; Vol. 36, No. 12. pp. 6303-6309.
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AU - Mantso, Theodora

AU - Sfakianos, Aristeidis P.

AU - Atkinson, Aithne

AU - Anestopoulos, Ioannis

AU - Mitsiogianni, Melina

AU - Botaitis, Sotirios

AU - Perente, Sebachedin

AU - Simopoulos, Constantinos

AU - Vasileiadis, Stavros

AU - Franco-Cruz, Rodrigo

AU - Pappa, Aglaia

AU - Panayiotidis, Mihalis I.

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