Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola

Linda L. Slivienski-Gebhardt, Jacques Izard, William A. Samsonoff, Ronald J. Limberger

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A new expression plasmid containing the fla operon promoter and a staphylococcal chloramphenicol resistance gene, was constructed to help assess the role of fliG in Treponema denticola motility. Deletion of fliG resulted in a nonmotile mutant with a markedly decreased number of flagellar filaments. Wild-type fliG genes from T. denticola and from Treponema pallidum were cloned into this expression plasmid. In both cases, the gene restored the ability of the mutant to gyrate its cell ends and enabled colony spreading in agarose. This shuttle plasmid enables high-level expression of genes in T. denticola and possesses an efficient selectable marker that provides a new tool for treponemal genetics.

Original languageEnglish (US)
Pages (from-to)5493-5497
Number of pages5
JournalInfection and immunity
Volume72
Issue number9
DOIs
StatePublished - Sep 1 2004

Fingerprint

Treponema denticola
Chloramphenicol Resistance
Plasmids
Genes
Treponema pallidum
Operon
Sepharose
Gene Expression

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola. / Slivienski-Gebhardt, Linda L.; Izard, Jacques; Samsonoff, William A.; Limberger, Ronald J.

In: Infection and immunity, Vol. 72, No. 9, 01.09.2004, p. 5493-5497.

Research output: Contribution to journalArticle

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