Developing defined and scalable 3D culture systems for culturing human pluripotent stem cells at high densities

Yuguo Lei, Daeun Jeong, Jifang Xiao, David V. Schaffer

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Human pluripotent stem cells (hPSCs) - including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) - are very promising candidates for cell therapies, tissue engineering, high throughput pharmacology screens, and toxicity testing. These applications require large numbers of high quality cells; however, scalable production of human pluripotent stem cells and their derivatives at a high density and under well-defined conditions has been a challenge. We recently reported a simple, efficient, fully defined, scalable, and good manufacturing practice (GMP) compatible 3D culture system based on a thermoreversible hydrogel for hPSC expansion and differentiation. Here, we describe additional design rationale and characterization of this system. For instance, we have determined that culturing hPSCs as a suspension in a liquid medium can exhibit lower volumetric yields due to cell agglomeration and possible shear force-induced cell loss. By contrast, using hydrogels as 3D scaffolds for culturing hPSCs reduces aggregation and may insulate from shear forces. Additionally, hydrogel-based 3D culture systems can support efficient hPSC expansion and differentiation at a high density if compatible with hPSC biology. Finally, there are considerable opportunities for future development to further enhance hydrogel-based 3D culture systems for producing hPSCs and their progeny.

Original languageEnglish (US)
Pages (from-to)172-183
Number of pages12
JournalCellular and Molecular Bioengineering
Volume7
Issue number2
DOIs
StatePublished - Jun 2014

Fingerprint

Pluripotent Stem Cells
Stem Cells
Stem cells
Cell culture
Hydrogel
Hydrogels
Cell
Cell Differentiation
Induced Pluripotent Stem Cells
Agglomeration
Cell Aggregation
Human
Culture
Cytology
Pharmacology
Tissue Engineering
Embryonic Stem Cells
Cell- and Tissue-Based Therapy
Scaffold
Cell Biology

Keywords

  • 3D culture system
  • Human embryonic stem cells
  • Induced pluripotent stem cells
  • Thermoreversible hydrogel

ASJC Scopus subject areas

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Developing defined and scalable 3D culture systems for culturing human pluripotent stem cells at high densities. / Lei, Yuguo; Jeong, Daeun; Xiao, Jifang; Schaffer, David V.

In: Cellular and Molecular Bioengineering, Vol. 7, No. 2, 06.2014, p. 172-183.

Research output: Contribution to journalArticle

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