Detwinning of Hemihedrally Twinned Crystals by the Least-Squares Method and its Application to a Crystal of Hydroxylamine Oxioreductase from Nitrosomanas europaea

Noriyuki Igarashi, Hideaki Moriyama, Tsohiyuki Mikami, Nobuo Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

With the development of methods to crystallize proteins to sizes suitable for X-ray analysis, structures of many proteins have been determined to elucidate the relationships between their structures and functions. However, crystals of biological macromolecules have been frequently found to be twinned by adherence of two crystal lattices. Some crystals show splitting of diffraction spots owing to the different tilts of the two lattices. Others pretend to be single crystals with no split spots, and their symmetries of intensity distribution vary with every data set. These latter have been called hemihedral, and in them the unique axes of the two crystals are exactly reversely parallel with each other. The observed intensities were found to be detwinned by the least-squares method after Britton's algorithm [Britton (1972). Acta Cryst. A28, 296-297], when several sets of intensity data were observed. Fortran programs have been successfully used to determine the scale factors and degrees of twinning from the data of hydroxylamine oxidoreductase. The analysis of data collected at various positions of one crystal of this enzyme suggested a heterogeneous distribution of twinning in the crystal.

Original languageEnglish (US)
Pages (from-to)362-367
Number of pages6
JournalJournal of Applied Crystallography
Volume30
Issue number3
DOIs
StatePublished - Jun 1 1997

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Hydroxylamine
hydroxylamine dehydrogenase
least squares method
Least-Squares Analysis
Crystals
crystals
Twinning
twinning
Proteins
Crystal lattices
X-Rays
proteins
Enzymes
X ray analysis
Crystal symmetry
Macromolecules
macromolecules
crystal lattices
enzymes
Diffraction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Detwinning of Hemihedrally Twinned Crystals by the Least-Squares Method and its Application to a Crystal of Hydroxylamine Oxioreductase from Nitrosomanas europaea. / Igarashi, Noriyuki; Moriyama, Hideaki; Mikami, Tsohiyuki; Tanaka, Nobuo.

In: Journal of Applied Crystallography, Vol. 30, No. 3, 01.06.1997, p. 362-367.

Research output: Contribution to journalArticle

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