Determining the Biosynthetic Sequence in the Early Steps of the Fumonisin Pathway by Use of Tree Gene-Disruption Mutants of Fusarium verticillioides

Ravi S. Bojja, Ronald Cerny, Robert H. Proctor, Liangcheng Du

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69 Citations (Scopus)

Abstract

Fumonisins are polyketide-derived mycotoxins produced by Fusarium verticillioides, a fungal pathogen of corn plants. Although a gene cluster for the biosynthesis of fumonisins has been cloned, the biosynthetic pathway is still not clear. We have used three gene-disrupted mutants, designated ΔFUM1, ΔFUM6, and ΔFUM8, to study the early steps of the pathway. Fumonisins were not produced in single-strain cultures of the ΔFUM1, ΔFUM6, and ΔFUM8 mutants. However, fumonisins were produced by ΔFUM1 or ΔFUM8 mutants when they were cocultured with the ΔFUM6 mutant. No fumonisins were produced when the ΔFUM1 and ΔFUM8 mutants were cocultured. These results suggest that the ΔFUM6 mutant produces a fumonisin intermediate that can be further metabolized by fumonisin biosynthetic enzymes in the ΔFUM1 and ΔFUM8 mutants. To isolate the potential intermediates produced by ΔFUM6, we followed a time course of cocultures of the ΔFUM1 and ΔFUM6 and the ΔFUM8 and ΔFUM6 mutants. Liquid chromatographic-mass spectrometric data suggested that metabolites having the general carbon skeleton of fumonisins with 1-4 hydroxyl groups were accumulated over a 7-day period. These results indicate that fumonisin biosynthesis starts with Fum1p-catalyzed carbon-chain assembly followed by the Fum8p-catalyzed alanine condensation. The resulting product then can be further oxidized by Fum6p and other enzymes.

Original languageEnglish (US)
Pages (from-to)2855-2860
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number10
DOIs
StatePublished - May 19 2004

Fingerprint

Fumonisins
fumonisins
Fusarium
gene targeting
Genes
mutants
Biosynthesis
Carbon
biosynthesis
Polyketides
Fusarium verticillioides
polyketides
Mycotoxins
carbon
Biosynthetic Pathways
Pathogens
coculture
Enzymes
Multigene Family
Metabolites

Keywords

  • Biosynthesis
  • Fumonisin
  • Fusarium verticillioides
  • Mycotoxin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{99d6bbd22b174529a8e6e328b8e241ce,
title = "Determining the Biosynthetic Sequence in the Early Steps of the Fumonisin Pathway by Use of Tree Gene-Disruption Mutants of Fusarium verticillioides",
abstract = "Fumonisins are polyketide-derived mycotoxins produced by Fusarium verticillioides, a fungal pathogen of corn plants. Although a gene cluster for the biosynthesis of fumonisins has been cloned, the biosynthetic pathway is still not clear. We have used three gene-disrupted mutants, designated ΔFUM1, ΔFUM6, and ΔFUM8, to study the early steps of the pathway. Fumonisins were not produced in single-strain cultures of the ΔFUM1, ΔFUM6, and ΔFUM8 mutants. However, fumonisins were produced by ΔFUM1 or ΔFUM8 mutants when they were cocultured with the ΔFUM6 mutant. No fumonisins were produced when the ΔFUM1 and ΔFUM8 mutants were cocultured. These results suggest that the ΔFUM6 mutant produces a fumonisin intermediate that can be further metabolized by fumonisin biosynthetic enzymes in the ΔFUM1 and ΔFUM8 mutants. To isolate the potential intermediates produced by ΔFUM6, we followed a time course of cocultures of the ΔFUM1 and ΔFUM6 and the ΔFUM8 and ΔFUM6 mutants. Liquid chromatographic-mass spectrometric data suggested that metabolites having the general carbon skeleton of fumonisins with 1-4 hydroxyl groups were accumulated over a 7-day period. These results indicate that fumonisin biosynthesis starts with Fum1p-catalyzed carbon-chain assembly followed by the Fum8p-catalyzed alanine condensation. The resulting product then can be further oxidized by Fum6p and other enzymes.",
keywords = "Biosynthesis, Fumonisin, Fusarium verticillioides, Mycotoxin",
author = "Bojja, {Ravi S.} and Ronald Cerny and Proctor, {Robert H.} and Liangcheng Du",
year = "2004",
month = "5",
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language = "English (US)",
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journal = "Journal of Agricultural and Food Chemistry",
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TY - JOUR

T1 - Determining the Biosynthetic Sequence in the Early Steps of the Fumonisin Pathway by Use of Tree Gene-Disruption Mutants of Fusarium verticillioides

AU - Bojja, Ravi S.

AU - Cerny, Ronald

AU - Proctor, Robert H.

AU - Du, Liangcheng

PY - 2004/5/19

Y1 - 2004/5/19

N2 - Fumonisins are polyketide-derived mycotoxins produced by Fusarium verticillioides, a fungal pathogen of corn plants. Although a gene cluster for the biosynthesis of fumonisins has been cloned, the biosynthetic pathway is still not clear. We have used three gene-disrupted mutants, designated ΔFUM1, ΔFUM6, and ΔFUM8, to study the early steps of the pathway. Fumonisins were not produced in single-strain cultures of the ΔFUM1, ΔFUM6, and ΔFUM8 mutants. However, fumonisins were produced by ΔFUM1 or ΔFUM8 mutants when they were cocultured with the ΔFUM6 mutant. No fumonisins were produced when the ΔFUM1 and ΔFUM8 mutants were cocultured. These results suggest that the ΔFUM6 mutant produces a fumonisin intermediate that can be further metabolized by fumonisin biosynthetic enzymes in the ΔFUM1 and ΔFUM8 mutants. To isolate the potential intermediates produced by ΔFUM6, we followed a time course of cocultures of the ΔFUM1 and ΔFUM6 and the ΔFUM8 and ΔFUM6 mutants. Liquid chromatographic-mass spectrometric data suggested that metabolites having the general carbon skeleton of fumonisins with 1-4 hydroxyl groups were accumulated over a 7-day period. These results indicate that fumonisin biosynthesis starts with Fum1p-catalyzed carbon-chain assembly followed by the Fum8p-catalyzed alanine condensation. The resulting product then can be further oxidized by Fum6p and other enzymes.

AB - Fumonisins are polyketide-derived mycotoxins produced by Fusarium verticillioides, a fungal pathogen of corn plants. Although a gene cluster for the biosynthesis of fumonisins has been cloned, the biosynthetic pathway is still not clear. We have used three gene-disrupted mutants, designated ΔFUM1, ΔFUM6, and ΔFUM8, to study the early steps of the pathway. Fumonisins were not produced in single-strain cultures of the ΔFUM1, ΔFUM6, and ΔFUM8 mutants. However, fumonisins were produced by ΔFUM1 or ΔFUM8 mutants when they were cocultured with the ΔFUM6 mutant. No fumonisins were produced when the ΔFUM1 and ΔFUM8 mutants were cocultured. These results suggest that the ΔFUM6 mutant produces a fumonisin intermediate that can be further metabolized by fumonisin biosynthetic enzymes in the ΔFUM1 and ΔFUM8 mutants. To isolate the potential intermediates produced by ΔFUM6, we followed a time course of cocultures of the ΔFUM1 and ΔFUM6 and the ΔFUM8 and ΔFUM6 mutants. Liquid chromatographic-mass spectrometric data suggested that metabolites having the general carbon skeleton of fumonisins with 1-4 hydroxyl groups were accumulated over a 7-day period. These results indicate that fumonisin biosynthesis starts with Fum1p-catalyzed carbon-chain assembly followed by the Fum8p-catalyzed alanine condensation. The resulting product then can be further oxidized by Fum6p and other enzymes.

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