Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry

Diana S. Aga, Seamus O'Connor, Steve Ensley, José O. Payero, Daniel D Snow, David Tarkalson

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

The persistence of manure-borne oxytetracycline in soil was investigated under field conditions. Soil cores were collected approximately once a month for over a period of two years and subsampled at depth increments of 0-5, 5-10, 10-15, 15-36, and 36-71 cm. Soil samples were analyzed by enzyme-linked immunosorbent assay (ELISA) and/or by liquid chromatography-mass spectrometry (LC-MS). Whereas LC-MS showed that oxytetracycline declined to <50% of its initial soil concentration after 3 weeks, ELISA showed that the total tetracyclines did not decline significantly 5 months after manure application. The differences between ELISA and LC-MS results are attributed to the broad cross-reactivity of the antibodies employed, which detect many structurally related tetracyclines, including their isomers and degradation products. Only trace amounts (≤ 1.0 μg/kg) of oxytetracycline were observed in the subsurface soil, and none was detected in water samples from field lysimeters, suggesting that oxytetracycline has low mobility in soil.

Original languageEnglish (US)
Pages (from-to)7165-7171
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number18
DOIs
StatePublished - Sep 7 2005

Fingerprint

Immunosorbents
soil enzymes
Manure
Manures
Liquid chromatography
Tetracycline
tetracycline
Liquid Chromatography
liquid chromatography
Mass spectrometry
oxytetracycline
Assays
Mass Spectrometry
Oxytetracycline
Soil
Enzyme-Linked Immunosorbent Assay
antibiotics
enzyme-linked immunosorbent assay
mass spectrometry
Anti-Bacterial Agents

Keywords

  • Antibiotics
  • ELISA
  • LC-MS
  • Manure
  • Mobility
  • Persistence

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry. / Aga, Diana S.; O'Connor, Seamus; Ensley, Steve; Payero, José O.; Snow, Daniel D; Tarkalson, David.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 18, 07.09.2005, p. 7165-7171.

Research output: Contribution to journalArticle

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