Determination of the DNA Sugar Pucker Using 13C NMR Spectroscopy

Rodolfo A. Santos, Pei Tang, Gerard S. Harbison

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Solid-state 13C NMR spectroscopy of a series of crystalline nucleosides and nucleotides allows direct measurement of the effect of the deoxyribose ring conformation on the carbon chemical shift. It is found that 3'-endo conformers have 3' and 5' chemical shifts significantly (5-10 ppm) upfield of comparable 3'-exo and 2'-endo conformers. The latter two conformers may be distinguished by smaller but still significant differences in the carbon chemical shifts at the C-2' and C-4' positions. High-resolution solid-state NMR of three modifications of fibrous calf thymus DNA shows that these trends are maintained in high-molecular-weight DNA and confirms that the major ring pucker in A-DNA is 3'-endo, while both B-DNA and C-DNA are largely 2'-endo. The data show that 13C NMR spectroscopy is a straightforward and useful probe of DNA ring pucker in both solution and the solid state.

Original languageEnglish (US)
Pages (from-to)9372-9378
Number of pages7
JournalBiochemistry
Volume28
Issue number24
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Chemical shift
Sugars
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
A-Form DNA
Carbon
B-Form DNA
Deoxyribose
DNA
DNA Probes
Nucleosides
Nucleotides
Molecular Weight
Conformations
Molecular weight
Nuclear magnetic resonance
Crystalline materials
Carbon-13 Magnetic Resonance Spectroscopy
calf thymus DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Determination of the DNA Sugar Pucker Using 13C NMR Spectroscopy. / Santos, Rodolfo A.; Tang, Pei; Harbison, Gerard S.

In: Biochemistry, Vol. 28, No. 24, 01.01.1989, p. 9372-9378.

Research output: Contribution to journalArticle

Santos, Rodolfo A. ; Tang, Pei ; Harbison, Gerard S. / Determination of the DNA Sugar Pucker Using 13C NMR Spectroscopy. In: Biochemistry. 1989 ; Vol. 28, No. 24. pp. 9372-9378.
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