Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization

Mark S. Schmidt, Thomas E. Prisinzano, Kevin Tidgewell, Wayne Harding, Eduardo R. Butelman, Mary J. Kreek, Daryl J. Murry

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.

Original languageEnglish (US)
Pages (from-to)221-225
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume818
Issue number2
DOIs
StatePublished - Apr 25 2005

Fingerprint

salvinorin A
Atmospheric Pressure
Body fluids
High performance liquid chromatography
Body Fluids
Atmospheric pressure
Ionization
High Pressure Liquid Chromatography
Metabolites
Macaca mulatta
Cerebrospinal fluid
Plasmas
Cerebrospinal Fluid
Negative ions
Urine
Guidelines
Ions
Derivatives

Keywords

  • APCT
  • Analysis
  • Body fluids
  • LC-MS
  • Metabolism
  • Plasma
  • Salvinorin
  • Salvinorin A
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization. / Schmidt, Mark S.; Prisinzano, Thomas E.; Tidgewell, Kevin; Harding, Wayne; Butelman, Eduardo R.; Kreek, Mary J.; Murry, Daryl J.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 818, No. 2, 25.04.2005, p. 221-225.

Research output: Contribution to journalArticle

Schmidt, Mark S. ; Prisinzano, Thomas E. ; Tidgewell, Kevin ; Harding, Wayne ; Butelman, Eduardo R. ; Kreek, Mary J. ; Murry, Daryl J. / Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2005 ; Vol. 818, No. 2. pp. 221-225.
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AU - Kreek, Mary J.

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