Determination of DNA cooperativity factor

B. R. Amirikyan, A. V. Vologodskii, Yuri L Lyubchenko

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The paper presents measurements of the difference in the melting temperature of a Co1E1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for 0.2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5×10-5 for the cooperatively factor σ. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

Original languageEnglish (US)
Pages (from-to)5469-5482
Number of pages14
JournalNucleic acids research
Volume9
Issue number20
DOIs
StatePublished - Oct 24 1981

Fingerprint

Nucleic Acid Denaturation
Melting
Freezing
DNA
Helix
Temperature
Reversibility
Coil
Melting point
Experimental Data
Factors

ASJC Scopus subject areas

  • Genetics

Cite this

Determination of DNA cooperativity factor. / Amirikyan, B. R.; Vologodskii, A. V.; Lyubchenko, Yuri L.

In: Nucleic acids research, Vol. 9, No. 20, 24.10.1981, p. 5469-5482.

Research output: Contribution to journalArticle

Amirikyan, B. R. ; Vologodskii, A. V. ; Lyubchenko, Yuri L. / Determination of DNA cooperativity factor. In: Nucleic acids research. 1981 ; Vol. 9, No. 20. pp. 5469-5482.
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