Determination of 3-methylhistidine by capillary electrophoresis.

Michael G Zeece, Q. Chu, S. J. Jones, T. L. Woods, W. J. Reville

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The post-translational methylation of histidine to form 3-methylhistidine (3MH) is a modification principally found in contractile proteins, and thus, the level of free 3MH has been used to monitor muscle protein turnover. This work describes procedures for the capillary electrophoretic separation and determination of the phenylthiohydantoin (PTH) derivative of 3MH using uncoated fused-silica capillaries. The procedure described here utilized UV detection and resulted in a linear standard curve in the range of 2-15 pmole, which is more sensitive than previously reported HPLC methods using fluorescent detection. In addition, good agreement for theoretical amounts of 3MH in hydrolyzed rabbit skeletal muscle actin and myofibril preparations from bovine skeletal muscle cells was found.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalJournal of capillary electrophoresis
Volume3
Issue number1
StatePublished - Jan 1 1996

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Muscle
Phenylthiohydantoin
Skeletal Muscle
Methylation
Fused silica
Contractile Proteins
Muscle Proteins
Myofibrils
Cells
Histidine
Silicon Dioxide
Muscle Cells
Derivatives
Proteins
Actins
High Pressure Liquid Chromatography
Rabbits
3-methylhistidine

ASJC Scopus subject areas

  • Biochemistry
  • Electrochemistry

Cite this

Zeece, M. G., Chu, Q., Jones, S. J., Woods, T. L., & Reville, W. J. (1996). Determination of 3-methylhistidine by capillary electrophoresis. Journal of capillary electrophoresis, 3(1), 55-59.

Determination of 3-methylhistidine by capillary electrophoresis. / Zeece, Michael G; Chu, Q.; Jones, S. J.; Woods, T. L.; Reville, W. J.

In: Journal of capillary electrophoresis, Vol. 3, No. 1, 01.01.1996, p. 55-59.

Research output: Contribution to journalArticle

Zeece, MG, Chu, Q, Jones, SJ, Woods, TL & Reville, WJ 1996, 'Determination of 3-methylhistidine by capillary electrophoresis.', Journal of capillary electrophoresis, vol. 3, no. 1, pp. 55-59.
Zeece MG, Chu Q, Jones SJ, Woods TL, Reville WJ. Determination of 3-methylhistidine by capillary electrophoresis. Journal of capillary electrophoresis. 1996 Jan 1;3(1):55-59.
Zeece, Michael G ; Chu, Q. ; Jones, S. J. ; Woods, T. L. ; Reville, W. J. / Determination of 3-methylhistidine by capillary electrophoresis. In: Journal of capillary electrophoresis. 1996 ; Vol. 3, No. 1. pp. 55-59.
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