Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli

M. Adamowicz, P. M. Kelley, K. W. Nickerson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was no apparent overlap between these 19 detergent (SDS) stress proteins and those of other known bacterial stress responses. The detergent stress stimulon is a distinct and independent stimulon. Its physiological relevance probably derives from the presence of bile salts in animal gastrointestinal tracts.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalJournal of bacteriology
Volume173
Issue number1
DOIs
StatePublished - Jan 1 1991

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Escherichia coli Proteins
Sodium Dodecyl Sulfate
Detergents
Shock
Electrophoresis, Gel, Two-Dimensional
Heat-Shock Proteins
Bile Acids and Salts
Gastrointestinal Tract
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli. / Adamowicz, M.; Kelley, P. M.; Nickerson, K. W.

In: Journal of bacteriology, Vol. 173, No. 1, 01.01.1991, p. 229-233.

Research output: Contribution to journalArticle

Adamowicz, M. ; Kelley, P. M. ; Nickerson, K. W. / Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli. In: Journal of bacteriology. 1991 ; Vol. 173, No. 1. pp. 229-233.
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