Detection of the free acid of bimatoprost in aqueous humor samples from human eyes treated with bimatoprost before cataract surgery

Carl B. Camras, Carol B Toris, Birgitta Sjoquist, Monica Milleson, John Olof Thorngren, Thomas W. Hejkal, Nehal Patel, Edward M. Barnett, Regina Smolyak, S. Fareed Hasan, Courtney Hellman, Jane L Meza, Martin B. Wax, Johan Stjernschantz

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

To determine whether bimatoprost is hydrolyzed to its free acid after topical application in humans in vivo. Prospective, masked, and vehicle controlled. Thirty-one eyes of 31 patients with cataracts. Beginning 7 days before scheduled cataract surgery, one eye of each patient was treated with bimatoprost 0.03% or vehicle once daily, with the last drop administered 2 to 12 hours before anterior chamber paracentesis before cataract surgery. In a masked fashion, aqueous humor specimens were assayed for bimatoprost and its free acid by high-pressure liquid chromatography and mass spectrometry. Detection of the free acid of bimatoprost in aqueous humor. Aqueous humor concentrations of the free acid of bimatoprost were 22.0±7.0 nmol/l (mean ± standard error of the mean, n = 12) and 7.0±4.6 nmol/l (n = 8) at 2 and 12 hours, respectively, and below the limit of detection after vehicle (n = 10). Concentrations of bimatoprost (amide) were 5.7±1.4 and 1.1±0.4 nmol/l at 2 and 12 hours, respectively, and undetectable after vehicle. After topical application of bimatoprost in humans, a sufficient concentration of its free acid, a potent FPprostanoid receptor agonist, is found in the aqueous humor to account for its ability to reduce intraocular pressure.

Original languageEnglish (US)
Pages (from-to)2193-2198
Number of pages6
JournalOphthalmology
Volume111
Issue number12
DOIs
StatePublished - Dec 1 2004

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Aqueous Humor
Cataract
Acids
Paracentesis
Anterior Chamber
Bimatoprost
Intraocular Pressure
Amides
Limit of Detection
Mass Spectrometry
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Detection of the free acid of bimatoprost in aqueous humor samples from human eyes treated with bimatoprost before cataract surgery. / Camras, Carl B.; Toris, Carol B; Sjoquist, Birgitta; Milleson, Monica; Thorngren, John Olof; Hejkal, Thomas W.; Patel, Nehal; Barnett, Edward M.; Smolyak, Regina; Hasan, S. Fareed; Hellman, Courtney; Meza, Jane L; Wax, Martin B.; Stjernschantz, Johan.

In: Ophthalmology, Vol. 111, No. 12, 01.12.2004, p. 2193-2198.

Research output: Contribution to journalArticle

Camras, CB, Toris, CB, Sjoquist, B, Milleson, M, Thorngren, JO, Hejkal, TW, Patel, N, Barnett, EM, Smolyak, R, Hasan, SF, Hellman, C, Meza, JL, Wax, MB & Stjernschantz, J 2004, 'Detection of the free acid of bimatoprost in aqueous humor samples from human eyes treated with bimatoprost before cataract surgery', Ophthalmology, vol. 111, no. 12, pp. 2193-2198. https://doi.org/10.1016/j.ophtha.2004.06.028
Camras, Carl B. ; Toris, Carol B ; Sjoquist, Birgitta ; Milleson, Monica ; Thorngren, John Olof ; Hejkal, Thomas W. ; Patel, Nehal ; Barnett, Edward M. ; Smolyak, Regina ; Hasan, S. Fareed ; Hellman, Courtney ; Meza, Jane L ; Wax, Martin B. ; Stjernschantz, Johan. / Detection of the free acid of bimatoprost in aqueous humor samples from human eyes treated with bimatoprost before cataract surgery. In: Ophthalmology. 2004 ; Vol. 111, No. 12. pp. 2193-2198.
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