Detection of retained microbubbles in carotid arteries with real-time low mechanical index imaging in the setting of endothelial dysfunction

Jeane M. Tsutsui, Feng Xie, Martin Cano, James Chomas, Patrick Phillips, Stanley J Radio, John Lof, Thomas Richard Porter

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We sought to determine if intravenously injected microbubbles would be retained by the carotid arteries (CAs) in the setting of endothelial dysfunction (ED) using a linear transducer equipped with a low mechanical index pulse sequence scheme (PSS). Microbubbles normally pass freely through large and small vessels but are retained in regions with ED. New high-frequency low mechanical index PSS can potentially be utilized to image these retained microbubbles. Intravenous albumin- and lipid-encapsulated microbubbles were administered in seven pigs while imaging the CAs before and after a 20% intralipid infusion to induce hypertriglyceridemia. The degree of microbubble retention was quantified by measuring endothelial acoustic intensity (AI) after clearance of free-flowing microbubbles. Microbubble adherence was also evaluated after selective balloon injury of the CAs. The CA diameter responses to acetylcholine were quantified. After induction of hypertriglyceridemia, adherence of albumin-encapsulated microbubbles was visually evident in all CAs, and endothelial AI increased significantly (p < 0.001 compared with baseline). The CA responses to acetylcholine went from vasodilation at baseline to vasoconstriction during hypertriglyceridemia. Endothelial AI also increased in the balloon-stretched vessels (p < 0.01 compared with uninjured vessels) after albumin-encapsulated microbubble injection, with a ring of microbubbles selectively adhering to the injured segment. This retention was not observed with lipid-encapsulated microbubbles. Scanning electron microscopy confirmed that albumin-coated microbubbles adhered to endothelial cells. Retention of intravenously injected albumin microbubbles occurs in the setting of both global and regional ED in large vessels and can be noninvasively imaged with high-frequency low mechanical index PSS.

Original languageEnglish (US)
Pages (from-to)1036-1046
Number of pages11
JournalJournal of the American College of Cardiology
Volume44
Issue number5
DOIs
StatePublished - Sep 1 2004

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Microbubbles
Carotid Arteries
Albumins
Hypertriglyceridemia
Acoustics
Acetylcholine
Carotid Artery Injuries
Lipids
Vasoconstriction
Transducers
Vasodilation
Electron Scanning Microscopy

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Detection of retained microbubbles in carotid arteries with real-time low mechanical index imaging in the setting of endothelial dysfunction. / Tsutsui, Jeane M.; Xie, Feng; Cano, Martin; Chomas, James; Phillips, Patrick; Radio, Stanley J; Lof, John; Porter, Thomas Richard.

In: Journal of the American College of Cardiology, Vol. 44, No. 5, 01.09.2004, p. 1036-1046.

Research output: Contribution to journalArticle

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