Detection of non-host viable contaminants in Pichia pastoris cultures and fermentation broths

Bradley A. Plantz, Jackie Andersen, Leonard A. Smith, Michael M. Meagher, Vicki L Schlegel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The ability to detect viable contaminants in cultures propagated from the original host-expression system ensures that the integrity and purity of seed banks, fermentation broths, and ultimately the final product are continually controlled and maintained. The method developed to detect such agents must be selective for a broad spectrum of microbes, which may be present at very low levels, while discriminating from the host organisms. Although Pichia pastoris strains are frequently used as cell lines for the expression of heterologous proteins, a method that is specific for monitoring culture purity has yet to be reported for this type of organism. An assay that is capable of recovering contaminating bacteria, fungi, and closely related yeast from cultures of P. pastoris at parts per million detection limits is described here.

Original languageEnglish (US)
Pages (from-to)643-650
Number of pages8
JournalJournal of Industrial Microbiology and Biotechnology
Volume30
Issue number11
DOIs
StatePublished - Nov 1 2003

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Pichia
Fungi
Yeast
Fermentation
Seed
Assays
Bacteria
Cells
Impurities
Proteins
Monitoring
Limit of Detection
Yeasts
Cell Line
Seed Bank

Keywords

  • Fermentation
  • Non-host contaminants
  • Pichia pastoris

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Detection of non-host viable contaminants in Pichia pastoris cultures and fermentation broths. / Plantz, Bradley A.; Andersen, Jackie; Smith, Leonard A.; Meagher, Michael M.; Schlegel, Vicki L.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 30, No. 11, 01.11.2003, p. 643-650.

Research output: Contribution to journalArticle

Plantz, Bradley A. ; Andersen, Jackie ; Smith, Leonard A. ; Meagher, Michael M. ; Schlegel, Vicki L. / Detection of non-host viable contaminants in Pichia pastoris cultures and fermentation broths. In: Journal of Industrial Microbiology and Biotechnology. 2003 ; Vol. 30, No. 11. pp. 643-650.
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