Detection of DNA adducts by 32P postlabeling following chronic exposure of rats to snuff

Raymond A. Smith, Terence Lawson, Sonny L. Johansson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A surgically created canal was used as a reservoir for instillation of snuff into the lower lip of rats for 10 weeks. DNA was isolated and digested with micrococcal nuclease/spleen phosphodiesterase. Polar DNA adducts were detected in all tissues examined when digests were fractionated by high performance liquid chromatography (HPLC), 32P postlabeled, 3' dephosphorylated and analyzed by two-dimensional thin layer chromatography (TLC). DNA adducts derived from aromatic carcinogens were not detected when digests were 32P postlabeled and analyzed by four-dimensional thin layer chromatography. These results suggest that non-aromatic agents initiate carcinogenesis following exposure to snuff. The adduction to DNA in organs of the gastrointestinal tract and the kidneys indicates that snuff usage results in systemic exposure to carcinogens and may contribute to the incidence of neoplasms in organs outside the oral cavity.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalCancer Letters
Volume121
Issue number1
DOIs
StatePublished - Dec 16 1997

Fingerprint

Smokeless Tobacco
DNA Adducts
spleen exonuclease
Thin Layer Chromatography
Carcinogens
Micrococcal Nuclease
DNA
Lip
Mouth
Gastrointestinal Tract
Carcinogenesis
High Pressure Liquid Chromatography
Kidney
Incidence
Neoplasms

Keywords

  • Adducts
  • Carcinogenesis
  • DNA
  • HPLC fractionation
  • P postlabeling
  • Snuff

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Detection of DNA adducts by 32P postlabeling following chronic exposure of rats to snuff. / Smith, Raymond A.; Lawson, Terence; Johansson, Sonny L.

In: Cancer Letters, Vol. 121, No. 1, 16.12.1997, p. 11-17.

Research output: Contribution to journalArticle

Smith, Raymond A. ; Lawson, Terence ; Johansson, Sonny L. / Detection of DNA adducts by 32P postlabeling following chronic exposure of rats to snuff. In: Cancer Letters. 1997 ; Vol. 121, No. 1. pp. 11-17.
@article{fc2157da63b94ad599c8c0cb2a7a5a5b,
title = "Detection of DNA adducts by 32P postlabeling following chronic exposure of rats to snuff",
abstract = "A surgically created canal was used as a reservoir for instillation of snuff into the lower lip of rats for 10 weeks. DNA was isolated and digested with micrococcal nuclease/spleen phosphodiesterase. Polar DNA adducts were detected in all tissues examined when digests were fractionated by high performance liquid chromatography (HPLC), 32P postlabeled, 3' dephosphorylated and analyzed by two-dimensional thin layer chromatography (TLC). DNA adducts derived from aromatic carcinogens were not detected when digests were 32P postlabeled and analyzed by four-dimensional thin layer chromatography. These results suggest that non-aromatic agents initiate carcinogenesis following exposure to snuff. The adduction to DNA in organs of the gastrointestinal tract and the kidneys indicates that snuff usage results in systemic exposure to carcinogens and may contribute to the incidence of neoplasms in organs outside the oral cavity.",
keywords = "Adducts, Carcinogenesis, DNA, HPLC fractionation, P postlabeling, Snuff",
author = "Smith, {Raymond A.} and Terence Lawson and Johansson, {Sonny L.}",
year = "1997",
month = "12",
day = "16",
doi = "10.1016/S0304-3835(97)00320-0",
language = "English (US)",
volume = "121",
pages = "11--17",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Detection of DNA adducts by 32P postlabeling following chronic exposure of rats to snuff

AU - Smith, Raymond A.

AU - Lawson, Terence

AU - Johansson, Sonny L.

PY - 1997/12/16

Y1 - 1997/12/16

N2 - A surgically created canal was used as a reservoir for instillation of snuff into the lower lip of rats for 10 weeks. DNA was isolated and digested with micrococcal nuclease/spleen phosphodiesterase. Polar DNA adducts were detected in all tissues examined when digests were fractionated by high performance liquid chromatography (HPLC), 32P postlabeled, 3' dephosphorylated and analyzed by two-dimensional thin layer chromatography (TLC). DNA adducts derived from aromatic carcinogens were not detected when digests were 32P postlabeled and analyzed by four-dimensional thin layer chromatography. These results suggest that non-aromatic agents initiate carcinogenesis following exposure to snuff. The adduction to DNA in organs of the gastrointestinal tract and the kidneys indicates that snuff usage results in systemic exposure to carcinogens and may contribute to the incidence of neoplasms in organs outside the oral cavity.

AB - A surgically created canal was used as a reservoir for instillation of snuff into the lower lip of rats for 10 weeks. DNA was isolated and digested with micrococcal nuclease/spleen phosphodiesterase. Polar DNA adducts were detected in all tissues examined when digests were fractionated by high performance liquid chromatography (HPLC), 32P postlabeled, 3' dephosphorylated and analyzed by two-dimensional thin layer chromatography (TLC). DNA adducts derived from aromatic carcinogens were not detected when digests were 32P postlabeled and analyzed by four-dimensional thin layer chromatography. These results suggest that non-aromatic agents initiate carcinogenesis following exposure to snuff. The adduction to DNA in organs of the gastrointestinal tract and the kidneys indicates that snuff usage results in systemic exposure to carcinogens and may contribute to the incidence of neoplasms in organs outside the oral cavity.

KW - Adducts

KW - Carcinogenesis

KW - DNA

KW - HPLC fractionation

KW - P postlabeling

KW - Snuff

UR - http://www.scopus.com/inward/record.url?scp=0030862272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030862272&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(97)00320-0

DO - 10.1016/S0304-3835(97)00320-0

M3 - Article

C2 - 9459168

AN - SCOPUS:0030862272

VL - 121

SP - 11

EP - 17

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -